Sparks, MD - BD Diagnostic Systems, a segment of BD (Becton, Dickinson and Company) announced that BBL™ CHROMagar™ Listeria has been validated by the AOAC™-Research Institute (RI) under the Performance Tested Methods ProgramSM for the analysis of raw ground beef, smoked salmon, lettuce and Brie cheese when using FDA BAM, USDA FSIS, AOAC and ISO methods. When used in conjunction with these methods, AOAC RI has validated that BBL CHROMagar Listeria provides an identification of L. monocytogenes/ivanovii with no confirmatory biochemical tests required.
An advantage BBL CHROMagar Listeria has over recommended traditional media, such as Modified Oxford and Oxford, is the ability to distinguish L. monocytogenes and L. ivanovii from other Listeria species. The high level of sensitivity and specificity of BBL CHROMagar Listeria reduces the amount of technologist time invested in identifying false positives from traditional media, reduces biochemical costs and improves turnaround time.
BBL CHROMagar Listeria utilizes a chromogenic substrate that produces a blue-green colony with halo when hydrolyzed by a specific enzyme. "Our evaluation of the new BBL CHROMagar Listeria showed a superior performance of the agar in its ability to differentiate Listeria monocytogenes and Listeria ivanovii from other Listeria species and background flora present in various food matrices and environmental samples, compared to non-chromogenic media commonly used for Listeria isolation", said Joseph Odumeru, Ph.D, Adjunct Professor, Department of Food Science at the University of Guelph in Ontario, Canada.
The efficient identification of Listeria monocytogenes is important in environmental samples. "Our experience with BBL CHROMagar Listeria, as applied to environmental samples, has been very positive. When comparing CHROMagar to Modified Oxford after prior enrichment, there was excellent agreement between the two media," said Lee-Ann Jaykus, Ph.D, Associate Professor of Food Science and Food Microbiology at NC State University. "Furthermore, interpretation based on colony morphology was easier with BBL CHROMagar Listeria. This saved time, media and labor. After presumptively positive Listeria and L. monocytogenes strains isolated from CHROMagar were further characterized, the species identities agreed with the initial CHROMagar interpretations. In short, our experience shows that BBL CHROMagar Listeria was an excellent pre-screen that greatly facilitated isolation and detection of Listeria as applied to environmental samples."
BBL™ CHROMagar™ Listeria is the latest formulation in the BBL™ CHROMagar™ media line to receive AOAC-RI certification. BBL CHROMagar Salmonella has been certified by AOAC-RI. Customers interested in more information on BBL™ CHROMagar™ Listeria, should call 1-800-638-8663, or contact their BD Diagnostic Systems representative.
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