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BBL™ IsoVitaleX™ Enrichment is a chemically defined supplement used as an additive to media for cultivation of nutritionally fastidious microorganisms.

Carpenter and Morton described an improved "chocolate" medium for the isolation of the gonococcus in 24 h.¹ The efficiency of this medium, GC Agar supplemented with hemoglobin and yeast concentrate, was demonstrated in a study of 12 media then in use for the isolation of this organism.² The medium was improved by replacing the yeast concentrate with BBL IsoVitaleX Enrichment, a chemically defined supplement developed specifically to aid the growth of gonococci, although it has broad application for other organisms, e.g., Haemophilus.^3,4

Thayer and Martin developed a selective "chocolate" medium, Thayer-Martin Selective Agar, for the primary isolation of Neisseria gonorrhoeae and N. meningitidis⁵ and improved it by using IsoVitaleX Enrichment as a nutritional supplement.³

Since then, IsoVitaleX Enrichment has been employed in improved media for the cultivation of pathogenic Neisseria, e.g., selective Modified Thayer-Martin Agar,⁶ Martin-Lewis Agar,⁷ and Transgrow Medium,⁸ as well as supplemented GC agar (GC Agar with IsoVitaleX Enrichment) for antimicrobial disc diffusion susceptibility testing of N. gonorrhoeae.⁹

Examine lyophilized and reconstituted enrichment for signs of deterioration as noted under "Product Deterioration".

Check performance of the complete medium with pure cultures of stable control organisms producing known desired reactions. The following cultures are recommended:

(For demonstrating suitability for use in antimicrobial disc diffusion susceptibility testing, refer to the reference.⁹)

Each vial of IsoVitaleX Enrichment is supplied with a vial of diluent (reconstituting solution).

For Laboratory Use

This Product Contains Dry Natural Rubber.

Observe aseptic techniques in the restoration and addition of this medium enrichment.