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V-C-A Inhibitor is an antibiotic mixture of vancomycin, colistin and anisomycin which is incorporated into culture media to permit the isolation of pathogenic Neisseria by inhibiting contaminating flora.

V-C-A-T Inhibitor is V-C-A Inhibitor plus trimethoprim to improve recovery of pathogenic Neisseria by increasing the selectivity of isolation media.

V-C-A Inhibitor was developed as an improved inhibitory supplement relative to the V-C-N (Vancomycin-Colistin-Nystatin) Inhibitor employed in Thayer-Martin-type selective media for the isolation of Neisseria gonorrhoeae and N. meningitidis.^1-4

It is employed in selective media, e.g., Martin-Lewis Agar,⁵ which is Chocolate Agar (BBL™ GC Agar Base or GC II Agar Base, Hemoglobin and IsoVitaleX™ Enrichment, a chemically defined supplement developed specifically to promote the growth of N. gonorrhoeae) supplemented with additional dextrose, V-C-A Inhibitor and trimethoprim lactate, to suppress the swarming of Proteus species.⁶ Because of its improved performance, it is particularly recommended for the isolation of pathogenic Neisseria.⁷

V-C-A-T Inhibitor may be used similarly as the V-C-A Inhibitor except that trimethoprim lactate has been included in the antibiotic mixture and the addition of the antibiotic separately is not required.

1. Examine lyophilized and restored supplement for signs of deterioration as noted under "Product Deterioration."

2. Check performance of the finished medium by inoculation with pure cultures of stable control organisms, producing known, desired reactions. The following cultures are recommended.

Formulae:

For Laboratory Use

This Product Contains Dry Natural Rubber.

V-C-A Inhibitor and V-C-A-T Inhibitor are for use in culture media and not for use in human or animal therapy.

Observe aseptic techniques in the restoration and addition of these media supplements.

Read directions for use.