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| Product Center | |
 Acridine Orange Stain, 250 mL bottle |
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| Cat. # | Desc. | Qty. | Unit |
| 212536 | Acridine Orange Stain, 250 mL bottle | 1 | EA |
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Acridine Orange Stain is recommended for use in the fluorescent microscopic detection of microorganisms in direct smears prepared from clinical and non-clinical materials. It is particularly useful in the rapid screening of normally sterile specimens, such as cerebrospinal fluid, where few organisms may be present and in the rapid examination of blood smears or smears containing proteinaceous material where differentiation of organisms from background material may be more difficult.
| Catalog # | Description | Quantity | Unit |
| 212536 | Acridine Orange Stain, 250 mL bottle | 1 | EA |
Acridine Orange Stain is recommended for use in the fluorescent microscopic detection of microorganisms in direct smears prepared from clinical and non-clinical materials. It is particularly useful in the rapid screening of normally sterile specimens, such as cerebrospinal fluid, where few organisms may be present and in the rapid examination of blood smears or smears containing proteinaceous material where differentiation of organisms from background material may be more difficult. Acridine Orange Stain is recommended for use in the fluorescent microscopic detection of microorganisms in direct smears prepared from clinical and non-clinical materials. It is particularly useful in the rapid screening of normally sterile specimens, such as cerebrospinal fluid, where few organisms may be present and in the rapid examination of blood smears or smears containing proteinaceous material where differentiation of organisms from background material may be more difficult. Fluorchromatic staining of microorganisms using acridine orange was first described by Strugger and Hilbrich in 1942. It has since been widely used in the examination of soil and water for microbial content. In 1975, Jones and Simon evaluated epifluorescent methods used in direct counts of aquatic bacteria and determined that acridine orange provided the best estimate of the bacterial population in lake, river and seawater samples.1 Acridine orange direct count (AODC) methodology has been used in the enumeration of landfill bacteria.2,3 Heidelberg et al. used AODC in a study of seasonal changes in marine bacterial populations and concluded that the acridine orange stain compared favorably to fluorescent oligonucleotide direct counting (FODC) procedures.4 Direct epifluoresent filter technique (DEFT) using acridine orange is specified in methods for the microbial examination of food and water.5,6,7,8 Acridine orange has also been used in clinical applications and its use in highlighting bacteria in blood cultures has become widely accepted. In 1980, McCarthy and Senne compared acridine orange staining with blind subcultures for the detection of positive blood cultures.9 Their results showed acridine orange staining to be a rapid, inexpensive alterative to blind subcultures. They also reported that the acridine orange stain appeared to be more sensitive than the Gram stain for detecting microorganims and was able to detect bacteria in concentrations of approximately 1 x 104 CFU (colony-forming units)/mL. Lauer, Reller and Mirret compared acridine orange with the Gram stain for detecting microorganisms in cerebrospinal fluid and other clinical materials.10 Their results were in agreement with those reported by McCarthy and Senne and showed acridine orange to be a simple, rapid staining procedure which was more sensitive than the Gram stain in detecting microorganisms in c linical materials. Acridine orange has also been used for the detection of Trichomonas vaginalis in vaginal smears,11 diagnosis of malaria12,13 and mycoplasmas.14 Fluorchromatic staining of microorganisms using acridine orange was first described by Strugger and Hilbrich in 1942. It has since been widely used in the examination of soil and water for microbial content. In 1975, Jones and Simon evaluated epifluorescent methods used in direct counts of aquatic bacteria and determined that acridine orange provided the best estimate of the bacterial population in lake, river and seawater samples.1 Acridine orange direct count (AODC) methodology has been used in the enumeration of landfill bacteria.2,3 Heidelberg et al. used AODC in a study of seasonal changes in marine bacterial populations and concluded that the acridine orange stain compared favorably to fluorescent oligonucleotide direct counting (FODC) procedures.4 Direct epifluoresent filter technique (DEFT) using acridine orange is specified in methods for the microbial examination of food and water.5,6,7,8 Acridine orange has also been used in clinical applications and its use in highlighting bacteria in blood cultures has become widely accepted. In 1980, McCarthy and Senne compared acridine orange staining with blind subcultures for the detection of positive blood cultures.9 Their results showed acridine orange staining to be a rapid, inexpensive alterative to blind subcultures. They also reported that the acridine orange stain appeared to be more sensitive than the Gram stain for detecting microorganims and was able to detect bacteria in concentrations of approximately 1 x 104 CFU (colony-forming units)/mL. Lauer, Reller and Mirret compared acridine orange with the Gram stain for detecting microorganisms in cerebrospinal fluid and other clinical materials.10 Their results were in agreement with those reported by McCarthy and Senne and showed acridine orange to be a simple, rapid staining procedure which was more sensitive than the Gram stain in detecting microorganisms in c linical materials. Acridine orange has also been used for the detection of Trichomonas vaginalis in vaginal smears,11 diagnosis of malaria12,13 and mycoplasmas.14 1. Examine the acridine orange staining solution for color and clarity. The solution should be clear, orange and without evidence of a precipitate. 2. Determine the pH of the solution. The pH should be 3.5 – 4.0. 3. Check the performance of the stain using 4 – 6 h Tryptic Soy Broth with 5% sheep blood cultures of the organisms indicated below. Prepare smears, one culture per slide, and proceed as described under Preparation, Staining and Examination of Smears.
For in vitro Diagnostic Use. Follow proper, established laboratory procedures in handling and disposing of infectious materials. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.
