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Difco™ Rose Bengal Antimicrobic Supplement C (chloramphenicol 0.05 g) is used with Difco Rose Bengal Base for selectively isolating and enumerating yeasts and molds from environmental materials and foodstuffs.

A number of methods have been described for the selective isolation of fungi from environmental materials and foodstuffs containing mixed populations of fungi and bacteria.

Rose Bengal Agar Base supplemented with Rose Bengal Antimicrobic Supplement C is a modification of the Rose Bengal Chlortetracycline Agar formula of Jarvis.¹ Instead of chlortetracycline, chloramphenicol is employed in this medium as a selective supplement. Of the antibiotics most frequently employed in media of neutral pH, chloramphenicol is recommended because of its heat stability and broad antibacterial spectrum. ²

Identity Specifications:

Lyophilized Appearance: White cake, may be dispersed.

Rehydrated Appearance: Colorless, clear.

Solubility: Soluble in 3 mL ethanol.

Prepare Difco Rose Bengal Agar per label directions. Inoculate using the pour plate technique (for Aspergillus niger, inoculate the surface of an agar slant) and incubate aerobically at 25–30°C for up to 7 days.

For Laboratory Use.

CAUSES IRRITATION. MAY CAUSE ALLERGIC SKIN REACTION. MAY CAUSE ALLERGIC RESPIRATORY REACTION. POSSIBLE CANCER HAZARD BASED ON TESTS WITH LABORATORY ANIMALS. OVEREXPOSURE MAY CREATE CANCER RISK. VERY TOXIC BY INHALATION AND IN CONTACT WITH SKIN.

Do not get in eyes, on skin, on clothing. Do not breathe dust, vapor, mist or gas, as applicable. Keep container closed. Use only with adequate ventilation. Wash thoroughly after handling.

First aid: IN CASE OF CONTACT, immediately flush eyes with plenty of water for at least 15 min. Call a physician. Flush skin with water. Wash clothing before reuse.

IF INHALED, remove to fresh air. If not breathing, give artificial respiration, preferably mouth-to-mouth. If breathing is difficult, give oxygen. Call a physician.

Caution: This product has been processed to render it free of microorganisms that would interfere with its intended use. The process has not been validated to the level required for an injectable drug or implantable device. The product has, however, passed a performance test for its intended use which included the demonstration of its freedom from interfering levels of microorganisms. Upon incorporation of this product into a testing system requiring sterility for proper performance, the entire system or a representative sample should be incubated to assure sterility before use.

Observe aseptic techniques and established precautions against microbiological hazards throughout all procedures. After use, prepared tubes, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.