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MacConkey I Agar
| Cat. # | Description | Qty. | Unit |
| 215197 | MacConkey I Agar | 20 | SP |
This medium is used in qualitative procedures for the isolation and cultivation of gram-negative enteric microorganisms from a variety of clinical and nonclinical specimens.
Meets USP/EP/JP performance specifications, where applicable.1-3
| Catalog # | Description | Quantity | Unit |
| 215197 | MacConkey I Agar | 20 | SP |
BBL™ Selective and Differential Prepared Plated Medium for Gram-Negative Microorganisms
MacConkey I Agar
Revision: 0707 Revision Date: 07/01/2007
This medium is used in qualitative procedures for the isolation and cultivation of gram-negative enteric microorganisms from a variety of clinical and nonclinical specimens.
Meets USP/EP/JP performance specifications, where applicable.1-3
MacConkey I Agar is a selective and differential medium for the detection of coliform organisms and enteric pathogens.4 Bile salts and crystal violet are employed as the selective agents. Neutral red is incorporated to differentiate lactose fermenters from lactose nonfermenters.
1. Examine plates for signs of deterioration as described under Product Deterioration.
2. Check performance by inoculating a representative sample of plates with pure cultures of stable control organisms that give known, desired reactions. The following test strains are recommended:
For clinical and other non-USP/EP/JP applications:
|
For USP/EP/JP applications*:
| Test Strain | Inoculum | Incubation | Expected Results |
| Escherichia coli ATCC 8739 |
<100 colony-forming units (CFU) |
30–35°C for 18–72 h |
Growth comparable to previously accepted lot of medium |
*For USP/EP/JP applications, inoculum level and incubation conditions are prescribed.
| Formula: | |||||
| Approximate Formula* Per Liter Purified Water | |||||
| Pancreatic Digest of Gelatin | 17.0 | g | |||
| Peptones (meat and casein) | 3.0 | g | |||
| Lactose | 10.0 | g | |||
| Bile Salts | 1.5 | g | |||
| Sodium Chloride | 5.0 | g | |||
| Neutral Red | 0.03 | g | |||
| Crystal Violet | 0.001 | g | |||
| Agar | 13.5 | g | |||
| *Adjusted and/or supplemented as required to meet performance criteria. | |||||
For in vitro Diagnostic Use.
If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.
Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.
