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TSI Agar is used for the differentiation of gram-negative enteric bacilli based on carbohydrate fermentation and the production of hydrogen sulfide.

TSI Agar is used for the determination of carbohydrate fermentation and hydrogen sulfide production in the identification of gram-negative bacilli.^1,2

Hajna developed the formulation for TSI Agar by adding sucrose to the double sugar (dextrose and lactose) formulation of Kligler Iron Agar.³ The addition of sucrose increased the sensitivity of the medium by facilitating the detection of sucrose-fermenting bacilli, as well as lactose and/or dextrose fermenters.

Carbohydrate fermentation is detected by the presence of gas and a visible color change (from red to yellow) of the pH indicator, phenol red. The production of hydrogen sulfide is indicated by the presence of a precipitate that blackens the medium in the butt of the tube.

See "Quality Control Procedures."

Quality Control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.

For in vitro Diagnostic Use.

Tubes with tight caps should be opened carefully to avoid injury due to breakage of glass.

Observe established precautions against microbiological hazards throughout all procedures. Prior to discarding, sterilize prepared tubes, specimen containers and other contaminated materials by autoclaving.