| Cat. # | Description | Qty. | Unit |
| 221169 | Chocolate II Agar (GC II Agar with Hemoglobin and IsoVitaleX™) | 20 | SP |
Chocolate II Agar is an improved medium for use in qualitative procedures for the isolation and cultivation of fastidious microorganisms, especially Neisseria and Haemophilus species, from a variety of clinical specimens.
| Catalog # | Description | Quantity | Unit |
| 221169 | Chocolate II Agar (GC II Agar with Hemoglobin and IsoVitaleX™) | 20 | SP |
Chocolate II Agar is an improved medium for use in qualitative procedures for the isolation and cultivation of fastidious microorganisms, especially Neisseria and Haemophilus species, from a variety of clinical specimens.
Carpenter and Morton described an improved medium for the isolation of the gonococcus in 24 h.1 The efficiency of this medium, GC Agar supplemented with hemoglobin and yeast concentrate, was demonstrated in a study of twelve media then in use for the isolation of this organism.2 The medium was improved by replacing the yeast concentrate with BBL IsoVitaleX Enrichment, a chemically defined supplement developed specially to aid the growth of gonococci, although it has broad application for other microorganisms, e.g., Haemophilus.3-5 Through careful selection and pretesting of raw materials, Chocolate II prepared plated medium promotes improved growth of gonococci and Haemophilus species. With most strains of N. gonorrhoeae, visible growth on primary isolation is seen after incubation of 18-24 h.
See "Quality Control Procedures."
Quality Control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.
| Chocolate II Agar (GC II Agar with Hemoglobin and IsoVitaleX™ Enrichment) | |||||
| Approximate Formula* Per Liter Purified Water | |||||
| Pancreatic Digest of Casein | 7.5 | g | |||
| Selected Meat Peptone | 7.5 | g | |||
| Corn Starch | 1.0 | g | |||
| Dipotassium Phosphate | 4.0 | g | |||
| Monopotassium Phosphate | 1.0 | g | |||
| Sodium Chloride | 5.0 | g | |||
| Agar | 12.0 | g | |||
| Hemoglobin | 10.0 | g | |||
| IsoVitaleX Enrichment | 10.0 | mL | |||
| *Adjusted and/or supplemented as required to meet performance criteria. | |||||
| IsoVitaleX™ Enrichment | |||||
| Approximate Formula* Per Liter Purified Water | |||||
| Vitamin B12 | 0.01 | g | |||
| L-Glutamine | 10.0 | g | |||
| Adenine | 1.0 | g | |||
| Guanine Hydrochloride | 0.03 | g | |||
| ρ-Aminobenzoic Acid | 0.013 | g | |||
| Nicotinamide Adenine Dinucleotide | 0.25 | g | |||
| Thiamine Pyrophosphate | 0.1 | g | |||
| Ferric Nitrate | 0.02 | g | |||
| Thiamine Hydrochloride | 0.003 | g | |||
| L-Cysteine Hydrochloride | 25.9 | g | |||
| L-Cystine | 1.1 | g | |||
| Dextrose | 100.0 | g | |||
| *Adjusted and/or supplemented as required to meet performance criteria. | |||||
For in vitro Diagnostic Use.
If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.
Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"6-9 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared plates, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.
Information shown on this page is a short summary extracted from the QC/PI Manual, available as a PDF under the Related Documents section of this page.
