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Mannitol Salt Agar
Cat. # Desc. Qty. Unit
221173 Mannitol Salt Agar 20 SP
Intended Use:

Mannitol Salt Agar is used for the selective isolation and enumeration of staphylococci from clinical and nonclinical materials.

Catalog # Description Quantity Unit
221173 Mannitol Salt Agar 20 SP

BBL™ Mannitol Salt Agar
Revision: 07    Revision Date: 09/01/2007   

Intended Use: «

Mannitol Salt Agar is used for the selective isolation and enumeration of staphylococci from clinical and nonclinical materials.

Intended Use: » Intended Use:

Mannitol Salt Agar is used for the selective isolation and enumeration of staphylococci from clinical and nonclinical materials.


Product Summary: «

Koch, in 1942, reported that only staphylococci grow on agar media containing 7.5% sodium chloride.1 Chapman further studied this phenomenon in greater detail and concluded that the addition of 7.5% sodium chloride to phenol red mannitol agar results in an improved medium for the isolation of plasma-coagulating staphylococci.2 This is the formulation supplied as BBL Mannitol Salt Agar.

Product Summary: » Product Summary:

Koch, in 1942, reported that only staphylococci grow on agar media containing 7.5% sodium chloride.1 Chapman further studied this phenomenon in greater detail and concluded that the addition of 7.5% sodium chloride to phenol red mannitol agar results in an improved medium for the isolation of plasma-coagulating staphylococci.2 This is the formulation supplied as BBL Mannitol Salt Agar.


User Quality Control: « User Quality Control:

See “Quality Control Procedures.”

Quality Control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory’s standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.


Reagents: « Reagents:
Mannitol Salt Agar
  Approximate Formula* Per Liter Purified Water
  Beef Extract  1.0   g
  Pancreatic Digest of Casein  5.0   g
  Peptic Digest of Animal Tissue  5.0   g
  Sodium Chloride  75.0   g
  D-Mannitol  10.0   g
  Phenol Red  0.025   g
  Agar  15.0   g
  *Adjusted and/or supplemented as required to meet performance criteria.

Warnings and Precautions: « Warnings and Precautions:

For in vitro Diagnostic Use.

If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.

Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"3-6 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared plates, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.



Information shown on this page is a short summary extracted from the QC/PI Manual, available as a PDF under the Related Documents section of this page.