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Salmonella Shigella Agar (SS Agar) is a differentially selective medium for the isolation of pathogenic enteric bacilli, especially those belonging to the genus Salmonella. This medium is not recommended for the primary isolation of Shigella.

The culture media which have been developed for the selection and differentiation of enteric microorganisms from clinical and nonclinical materials inhibit the growth of gram-positive species to a varying degree due to the presence of either pure bile salts, mixtures of bile salts or dyes. One of the formulations currently used in the plating of samples for the detection of enteric pathogens is Salmonella Shigella Agar, which is an example of the media containing bile salt mixtures. This medium is essentially a modification of the Desoxycholate-Citrate Agar described by Leifson.¹

See "Quality Control Procedures."

Quality Control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.

For in vitro Diagnostic Use.

If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.

Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"^2-5 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared plates, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.