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Trypticase Soy Agar with 5% or 10% Sheep Blood is used for cultivating fastidious microorganisms and for the visualization of hemolytic reactions produced by many bacterial species. Plates labeled "deep fill" contain an additional volume of medium to reduce the effects of drying during prolonged incubation.

The nutritional composition of Trypticase Soy Agar has made it a popular medium, both unsupplemented and as a base for media containing blood. Trypticase Soy Agar with 5% or 10% Sheep Blood is extensively used for the recovery and cultivation of fastidious microbial species and for the determination of hemolytic reactions which are important differentiating characteristics for bacteria, especially Streptococcus species.

See "Quality Control Procedures."

Quality Control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.

For in vitro Diagnostic Use.

If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.

Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"^3-6 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared plates, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.