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Phenylethyl Alcohol Agar with 5% Sheep Blood



Cat. # Description Qty. Unit
221277 Phenylethyl Alcohol Agar with 5% Sheep Blood
100 SP

Intended Use:

Phenylethyl Alcohol Agar with 5% Sheep Blood is a selective medium for the isolation of gram-positive organisms, particularly gram-positive cocci, from specimens of mixed gram-positive and gram-negative flora. The medium should not be used for determination of hemolytic reactions since atypical reactions may be observed.


Catalog # Description Quantity Unit
221277 Phenylethyl Alcohol Agar with 5% Sheep Blood
100 SP

BBL™ Phenylethyl Alcohol Agar with 5% Sheep Blood
Revision: 07     Revision Date: 05/01/2006    

Intended Use:

Phenylethyl Alcohol Agar with 5% Sheep Blood is a selective medium for the isolation of gram-positive organisms, particularly gram-positive cocci, from specimens of mixed gram-positive and gram-negative flora. The medium should not be used for determination of hemolytic reactions since atypical reactions may be observed.



Product Summary:

After having noted that phenylethyl alcohol exhibited a marked inhibitory effect on gram-negative bacteria with only slight effect on gram-positive organisms, Lilley and Brewer incorporated the chemical in an infusion agar base as a selective agent for the isolation of gram-positive bacteria.1 Phenylethyl Alcohol with 5% Sheep Blood is used in the microbiology laboratory to inhibit gram-negative bacteria, particularly Proteus, in specimens containing a mixed bacterial flora.



User Quality Control:

See "Quality Control Procedures."

Quality Control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.


Reagents:

Phenylethyl Alcohol Agar with 5% Sheep Blood
  Approximate Formula* Per Liter Purified Water
  Pancreatic Digest of Casein  15.0   g
  Papaic Digest of Soybean Meal  5.0   g
  Sodium Chloride  5.0   g
  ß-Phenylethyl Alcohol  2.5   g
  Agar  15.0   g
  Sheep Blood, defibrinated  5%  
  *Adjusted and/or supplemented as required to meet performance criteria.


Warnings and Precautions:

For in vitro Diagnostic Use.

If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.

Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"3-6 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared plates, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.



Information shown on this page is a short summary extracted from the QC/PI Manual, available as a PDF under the Related Documents section of this page.