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Thayer Martin Selective Agar
| Cat. # | Description | Qty. | Unit |
| 221282 | Thayer Martin Selective Agar | 100 | SP |
Thayer-Martin Selective Agar is used for the isolation of pathogenic Neisseria from specimens containing mixed flora of bacteria and fungi.
| Catalog # | Description | Quantity | Unit |
| 221282 | Thayer Martin Selective Agar | 100 | SP |
BBL™ Thayer-Martin Selective Agar
Revision: 07 Revision Date: 09/01/2007
Thayer-Martin Selective Agar is used for the isolation of pathogenic Neisseria from specimens containing mixed flora of bacteria and fungi.
Carpenter and Morton described an improved medium for the isolation of the gonococcus in 24 h.1 The efficiency of this medium, GC Agar supplemented with hemoglobin and yeast concentrate, was demonstrated in a study of twelve media then in use for the isolation of this organism.2 The medium was improved by replacing the yeast concentrate with BBL IsoVitaleX™ Enrichment, a chemically defined supplement developed specifically to aid the growth of gonococci, although it has broad application for other microorganisms, e.g., Haemophilus.3-5
Thayer-Martin Selective Agar was developed for the primary isolation of N. gonorrhoeae and N. meningitidis from specimens containing mixed flora taken from the throat, vagina, rectum, and urethra.4,6,7 Consisting of Chocolate II Agar with vancomycin, colistin and nystatin, it is formulated to minimize the overgrowth of gonococci and meningococci by contaminants, to suppress the growth of saprophytic Neisseria species and to enhance the growth of pathogenic Neisseria.
See “Quality Control Procedures.”
Quality Control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory’s standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.
| Thayer-Martin Selective Agar | |||||
| Approximate Formula* Per Liter Purified Water | |||||
| Pancreatic Digest of Casein | 7.5 | g | |||
| Selected Meat Peptone | 7.5 | g | |||
| Corn Starch | 1.0 | g | |||
| Dipotassium Phosphate | 4.0 | g | |||
| Monopotassium Phosphate | 1.0 | g | |||
| Sodium Chloride | 5.0 | g | |||
| Agar | 12.0 | g | |||
| Hemoglobin | 10.0 | g | |||
| IsoVitaleX Enrichment | 10.0 | mL | |||
| V-C-N Inhibitor | 10.0 | mL | |||
| *Adjusted and/or supplemented as required to meet performance criteria. | |||||
| IsoVitaleX Enrichment | |||||
| Approximate Formula* Per Liter Purified Water | |||||
| Vitamin B12 | 0.01 | g | |||
| L-Glutamine | 10.0 | g | |||
| Adenine | 1.0 | g | |||
| Guanine Hydrochloride | 0.03 | g | |||
| ρ-Aminobenzoic Acid | 0.013 | g | |||
| Nicotinamide Adenine Dinucleotide | 0.25 | g | |||
| Thiamine Pyrophosphate | 0.1 | g | |||
| Ferric Nitrate | 0.02 | g | |||
| Thiamine Hydrochloride | 0.003 | g | |||
| L-Cysteine Hydrochloride | 25.9 | g | |||
| L-Cystine | 1.1 | g | |||
| Dextrose | 100.0 | g | |||
| *Adjusted and/or supplemented as required to meet performance criteria. | |||||
| V-C-N Inhibitor | |||||
| Formula Per One Milliliter Restored Solution | |||||
| Vancomycin | 300 | μg | |||
| Colistin | 750 | μg | |||
| Nystatin | 1250 | units | |||
For in vitro Diagnostic Use.
If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.
Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"8-11 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared plates, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.
Information shown on this page is a short summary extracted from the QC/PI Manual, available as a PDF under the Related Documents section of this page.
