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Trypticase Soy Agar is used for the isolation and cultivation of non-fastidious and fastidious microorganisms. It is not the medium of choice for anaerobes.

The nutritional composition of Trypticase Soy Agar has made it a popular medium for many years. It is the medium specified as Soybean-Casein Digest Agar Medium in The United States Pharmacopeia for the total aerobic microbial count portion of the microbial limit testing procedures.¹ The medium is used for a multitude of purposes including maintenance of stock cultures, plate counting, isolation of microorganisms from a variety of specimen types and as a base for media containing blood.^2-4 It is included in the compendia of methods for the examination of water, wastewater and foods.^5,6

See "Quality Control Procedures."

Quality Control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.

For in vitro Diagnostic Use.

If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.

Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"^7-10 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared plates, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.