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XLD Agar
Cat. # Desc. Qty. Unit
221284 XLD Agar 100 SP
Intended Use:

XLD Agar is the complete Xylose Lysine Desoxycholate Agar recommended for isolation and differentiation of enteric pathogens, especially Shigella species.

Catalog # Description Quantity Unit
221284 XLD Agar 100 SP

BBL™ XLD Agar
Revision: 09    Revision Date: 09/01/2007   

Intended Use: «

XLD Agar is the complete Xylose Lysine Desoxycholate Agar recommended for isolation and differentiation of enteric pathogens, especially Shigella species.

Intended Use: » Intended Use:

XLD Agar is the complete Xylose Lysine Desoxycholate Agar recommended for isolation and differentiation of enteric pathogens, especially Shigella species.


Product Summary: «

A wide variety of media have been developed to aid in the selective isolation and differentiation of enteric pathogens. Due to the large number of different microbial species and strains with varying nutritional requirements and chemical resistance patterns, investigators have developed various formulae to meet general as well as specific needs relative to isolation and identification of these microorganisms.

XLD Agar was developed by Taylor in order to increase the efficiency of the isolation and identification of enteric pathogens, particularly Shigella.1 The pathogens are differentiated not only from the nonpathogenic lactose fermenters but also from many nonpathogens which do not ferment lactose or sucrose. Additionally, the medium was formulated to increase the frequency of growth of the more fastidious pathogens,1 which in other formulations often failed to grow due to the inclusion of excessively toxic inhibitors. The results obtained in a number of clinical evaluations have supported the claim for the relatively high efficiency of XLD Agar in the primary isolation of Shigella and Salmonella.2-6

Product Summary: » Product Summary:

A wide variety of media have been developed to aid in the selective isolation and differentiation of enteric pathogens. Due to the large number of different microbial species and strains with varying nutritional requirements and chemical resistance patterns, investigators have developed various formulae to meet general as well as specific needs relative to isolation and identification of these microorganisms.

XLD Agar was developed by Taylor in order to increase the efficiency of the isolation and identification of enteric pathogens, particularly Shigella.1 The pathogens are differentiated not only from the nonpathogenic lactose fermenters but also from many nonpathogens which do not ferment lactose or sucrose. Additionally, the medium was formulated to increase the frequency of growth of the more fastidious pathogens,1 which in other formulations often failed to grow due to the inclusion of excessively toxic inhibitors. The results obtained in a number of clinical evaluations have supported the claim for the relatively high efficiency of XLD Agar in the primary isolation of Shigella and Salmonella.2-6


User Quality Control: « User Quality Control:

See "Quality Control Procedures."

Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.


Reagents: « Reagents:
XLD Agar
  Approximate Formula* Per Liter Purified Water
  Xylose  3.5   g
  L-Lysine  5.0   g
  Lactose  7.5   g
  Saccharose  7.5   g
  Sodium Chloride  5.0   g
  Yeast Extract  3.0   g
  Phenol Red  0.08   g
  Sodium Desoxycholate  2.5   g
  Sodium Thiosulfate  6.8   g
  Ferric Ammonium Citrate  0.8   g
  Agar  13.5   g
  *Adjusted and/or supplemented as required to meet performance criteria.

Warnings and Precautions: « Warnings and Precautions:

For in vitro Diagnostic Use.

If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.

Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"7-10 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared plates, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.



Information shown on this page is a short summary extracted from the QC/PI Manual, available as a PDF under the Related Documents section of this page.