United StatesProduct Center
OF Medium with Xylose
| Cat. # | Description | Qty. | Unit |
| 221338 | OF Medium with Xylose | 10 | SP |
OF (Oxidation Fermentation) media are used for the determination of oxidative and fermentative metabolism of carbohydrates by gram-negative rods on the basis of acid reaction in either the open or closed system.
| Catalog # | Description | Quantity | Unit |
| 221338 | OF Medium with Xylose | 10 | SP |
BBL™ OF Basal Medium
BBL™ OF Medium with Lactose
BBL™ OF Medium with Maltose
BBL™ OF Medium with Mannitol
BBL™ OF Medium with Sucrose
BBL™ OF Medium with Xylose
Revision: 00 Revision Date: 01/01/2006
OF (Oxidation Fermentation) media are used for the determination of oxidative and fermentative metabolism of carbohydrates by gram-negative rods on the basis of acid reaction in either the open or closed system.
OF Medium was developed by Hugh and Leifson who described the taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by gram-negative bacteria.1 They showed that when an organism is inoculated into two tubes of OF Basal Medium containing a carbohydrate and the medium in one of the tubes is covered with melted petrolatum prior to incubation, the patterns of metabolism are of differential significance. Oxidative organisms only produce an acid reaction in the open tube with little or no growth and no acid formation in the covered tube. Fermentative organisms will produce an acid reaction in both types of tubes.
Changes in the covered agar are considered to be due to true fermentation, while changes in the open tubes are due to oxidative utilization of the carbohydrate present. If the carbohydrate is not utilized by either method, there is no acid production in either tube.
See "Quality Control Procedures."
Quality Control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.
A single electrode of sufficiently small size to fit into the tubes should be used to determine the pH potentiometrically of tubed media. The tip of the electrode should be positioned in the central portion of the agar mass in semisolid media.
| OF Basal Medium | |||||
| Approximate Formula* Per Liter Purified Water | |||||
| Pancreatic Digest of Casein | 2.0 | g | |||
| Sodium Chloride | 5.0 | g | |||
| Dipotassium Phosphate | 0.3 | g | |||
| Agar | 2.5 | g | |||
| Bromthymol Blue | 0.03 | g | |||
| *Adjusted and/or supplemented as required to meet performance criteria. | |||||
OF Medium with Lactose, Maltose, Mannitol, Sucrose or Xylose contains the above ingredients with, per liter, 10.0 g of the specified carbohydrate.
For in vitro Diagnostic Use.
Tubes with tight caps should be opened carefully to avoid injury due to breakage of glass.
Observe aseptic techniques and established precautions against microbiological hazards throughout all procedures. After use, prepared tubes, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.
Information shown on this page is a short summary extracted from the QC/PI Manual, available as a PDF under the Related Documents section of this page.
