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Acetate Differential Agar Slants
Cat. # Desc. Qty. Unit
221375 Acetate Differential Agar Slants 10 SP
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Intended Use:

Acetate Differential Agar is used for the differentiation of Shigella species from Escherichia coli.

Catalog # Description Quantity Unit
221375 Acetate Differential Agar Slants 10 SP

BBL™ Acetate Differential Agar Slants
Revision: 06    Revision Date: 05/01/2006   

Intended Use: «

Acetate Differential Agar is used for the differentiation of Shigella species from Escherichia coli.

Intended Use: » Intended Use:

Acetate Differential Agar is used for the differentiation of Shigella species from Escherichia coli.


Product Summary: «

Organic acids have been used widely as an aid to the differentiation of Enterobacteriaceae, usually in formulae that contained organic nitrogen sources. Most bacteria, however, can use citrate and acetate in the presence of organic nitrogen.

The citrate media of Koser1 and Simmons2 were free of organic nitrogen and, therefore, were a true measure of citrate utilization. In a further extension of this approach, Trabulsi and Ewing developed Acetate Differential Agar, a chemically defined medium utilizing sodium acetate which enables the differentiation of Shigella species from E. coli.3,4 Their basal medium was Simmons Citrate Agar in which sodium acetate was substituted for sodium citrate.

Product Summary: » Product Summary:

Organic acids have been used widely as an aid to the differentiation of Enterobacteriaceae, usually in formulae that contained organic nitrogen sources. Most bacteria, however, can use citrate and acetate in the presence of organic nitrogen.

The citrate media of Koser1 and Simmons2 were free of organic nitrogen and, therefore, were a true measure of citrate utilization. In a further extension of this approach, Trabulsi and Ewing developed Acetate Differential Agar, a chemically defined medium utilizing sodium acetate which enables the differentiation of Shigella species from E. coli.3,4 Their basal medium was Simmons Citrate Agar in which sodium acetate was substituted for sodium citrate.


User Quality Control: « User Quality Control:

See "Quality Control Procedures."

Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.


Reagents: « Reagents:
Acetate Differential Agar
  Approximate Formula* Per Liter Purified Water
  Sodium Acetate  2.0   g
  Magnesium Sulfate  0.2   g
  Sodium Chloride  5.0   g
  Monoammonium Phosphate  1.0   g
  Dipotassium Phosphate  1.0   g
  Bromthymol Blue  0.08   g
  Agar  14.8   g
  *Adjusted and/or supplemented as required to meet performance criteria.

Warnings and Precautions: « Warnings and Precautions:

For in vitro Diagnostic Use.

Tubes with tight caps should be opened carefully to avoid injury due to breakage of glass.

Observe aseptic techniques and established precautions against microbiological hazards throughout all procedures. After use, prepared tubes, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.



Information shown on this page is a short summary extracted from the QC/PI Manual, available as a PDF under the Related Documents section of this page.