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Brucella Agar with 5% Horse Blood is used in qualitative procedures for the isolation and cultivation of fastidious and nonfastidious microorganisms from a variety of clinical and nonclinical specimens.

Brucella Agar was developed for the cultivation of Brucella species from diagnostic specimens such as blood, and from foods and other potentially contaminated material. Brucella Agar with 5% Horse Blood plates are particularly useful for the cultivation of the more fastidious aerobic and anaerobic microorganisms including streptococci, pneumococci, Listeria, Neisseria meningitidis and Haemophilus influenzae.

See "Quality Control Procedures."

Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.

For in vitro Diagnostic Use.

If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.

Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"^3-6 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared plates, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.

Biosafety Level 2 practices, containment equipment and facilities are recommended for activities with clinical specimens of human or animal origin containing or potentially containing pathogenic Brucella spp. Biosafety Level 3 practices, containment equipment and facilities are recommended for all manipulations of cultures of the pathogenic Brucella spp. and for experimental animal studies.⁵