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Trypticase Soy Broth conforms with specifications of The United States Pharmacopeia (USP).

Trypticase Soy Broth (Soybean-Casein Digest Medium) is a general-purpose medium used in qualitative procedures for the cultivation of fastidious and nonfastidious microorganisms from a variety of clinical and nonclinical specimens.

Trypticase Soy Broth with 6.5% Sodium Chloride is used to differentiate Enterococcus species from the Streptococcus bovis group of streptococci.

Trypticase Soy Broth (TSB) is a nutritious medium that will support the growth of a wide variety of microorganisms, including common aerobic, facultative and anaerobic bacteria and fungi.^1-4 Because of its capacity for growth promotion, this formulation is included in the USP as a sterility test medium.⁵

TSB is also recommended, because of growth promotion, for use as the inoculum broth for disc diffusion and agar-dilution antimicrobial susceptibility testing as standardized by the Clinical and Laboratory Standards Institute (formerly NCCLS).^6,7

Trypticase Soy Broth with 6.5% Sodium Chloride is used to differentiate the enterococcal species from the S. bovis group of streptococci by the 6.5% NaCl tolerance test.⁸

See "Quality Control Procedures."

Quality Control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI guidance and CLIA regulations for appropriate Quality Control practices.

A single electrode of sufficiently small size to fit into the tubes should be used to determine the pH potentiometrically of tubed media. The tip of the electrode should be placed below the surface of broth media (Trypticase Soy Broth only).

Trypticase Soy Broth with 6.5% Sodium Chloride contains 60 g/L of sodium chloride in addition to the ingredients listed above.

For in vitro Diagnostic Use.

Caution should be exercised in reporting direct Gram stain and/or other direct microbiological stain results on tissue specimens processed with this medium due to the possible presence of nonviable organisms in the culture medium.

Tubes with tight caps should be opened carefully to avoid injury due to breakage of glass.

Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"^9-12 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared tubes, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.