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GN Broth is used for the selective enrichment of Salmonella and Shigella.

GN (Gram Negative) Broth was developed by Hajna as an enrichment medium for the recovery of Salmonella and Shigella from clinical specimens.^1,2 Croft and Miller succeeded in isolating more Shigella strains by use of this medium, rather than by direct streaking.³ Taylor and Schelhart reported that GN Broth enhanced the isolation of enteric pathogens, producing a 53% increase in Shigella and 36% increase in Salmonella as compared to direct streaking.⁴

GN Broth currently is recommended for use in the microbiological examination of foods.⁵

See "Quality Control Procedures."

Quality Control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.

For in vitro Diagnostic Use.

Tubes with tight caps should be opened carefully to avoid injury due to breakage of glass.

Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"^6-9 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared tubes, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.