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BHI (Brain Heart Infusion) Broth, 5 mL
Cat. # Desc. Qty. Unit
221812 BHI (Brain Heart Infusion) Broth, 5 mL 10 SP
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Intended Use:

Brain Heart Infusion (BHI) is a general-purpose liquid medium used in the cultivation of fastidious and nonfastidious microorganisms, including aerobic and anaerobic bacteria, from a variety of clinical and nonclinical materials.

The broth medium which contains 6.5% sodium chloride is used to differentiate the enterococci from nonenterococcal group D streptococci.

Catalog # Description Quantity Unit
221812 BHI (Brain Heart Infusion) Broth, 5 mL 10 SP

BBL™ Brain Heart Infusion
BBL™ Brain Heart Infusion with 6.5% Sodium Chloride
Revision: 09    Revision Date: 02/01/2007   

Intended Use: «

Brain Heart Infusion (BHI) is a general-purpose liquid medium used in the cultivation of fastidious and nonfastidious microorganisms, including aerobic and anaerobic bacteria, from a variety of clinical and nonclinical materials.

The broth medium which contains 6.5% sodium chloride is used to differentiate the enterococci from nonenterococcal group D streptococci.

Intended Use: » Intended Use:

Brain Heart Infusion (BHI) is a general-purpose liquid medium used in the cultivation of fastidious and nonfastidious microorganisms, including aerobic and anaerobic bacteria, from a variety of clinical and nonclinical materials.

The broth medium which contains 6.5% sodium chloride is used to differentiate the enterococci from nonenterococcal group D streptococci.


Product Summary: «

BHI Broth is used for the cultivation of a wide variety of microorganisms, including bacteria, yeasts and molds.1

BHI with 6.5% Sodium Chloride is used to differentiate the enterococci (e.g., E. faecalis, E. faecium, E. durans and E. avium) from the nonenterococcal species (S. bovis and S. equinus) by the 6.5% salt tolerance test.2

Product Summary: » Product Summary:

BHI Broth is used for the cultivation of a wide variety of microorganisms, including bacteria, yeasts and molds.1

BHI with 6.5% Sodium Chloride is used to differentiate the enterococci (e.g., E. faecalis, E. faecium, E. durans and E. avium) from the nonenterococcal species (S. bovis and S. equinus) by the 6.5% salt tolerance test.2


User Quality Control: « User Quality Control:

See “Quality Control Procedures.”

Quality Control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory’s standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.


Reagents: « Reagents:
Brain Heart Infusion
  Approximate Formula* Per Liter Purified Water
  Brain Heart, Infusion from (solids)  6.0   g
  Peptic Digest of Animal Tissue  6.0   g
  Sodium Chloride  5.0   g
  Dextrose  3.0   g
  Pancreatic Digest of Gelatin  14.5   g
  Disodium Phosphate  2.5   g
  *Adjusted and/or supplemented as required to meet performance criteria.

Brain Heart Infusion with 6.5% Sodium Chloride contains 60 g/L of sodium chloride in addition to the ingredients listed above.


Warnings and Precautions: « Warnings and Precautions:

For in vitro Diagnostic Use.

Tubes with tight caps should be opened carefully to avoid injury due to breakage of glass.

Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"3-6 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared tubes, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.



Information shown on this page is a short summary extracted from the QC/PI Manual, available as a PDF under the Related Documents section of this page.