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Bile Esculin Agar
| Cat. # | Description | Qty. | Unit |
| 221838 | Bile Esculin Agar | 10 | SP |
Bile Esculin Agar is used to differentiate enterococci and the Streptococcus bovis group from other streptococci.1,2
| Catalog # | Description | Quantity | Unit |
| 221838 | Bile Esculin Agar | 10 | SP |
BBL™ Bile Esculin Agar
Revision: 10 Revision Date: 04/01/2006
Bile Esculin Agar is used to differentiate enterococci and the Streptococcus bovis group from other streptococci.1,2
Rochaix noted the value of esculin hydrolysis in the identification of enterococci.3 The enterococci were able to split esculin, but other streptococci could not. Meyer and Schonfeld incorporated bile into the esculin medium and showed that 61 of 62 enterococci were able to grow and split esculin, whereas the other streptococci could not.4 Swan used an esculin medium containing 40% bile salts and reported that a positive reaction on the bile esculin medium correlated with a serological group D precipitin reaction.5
See "Quality Control Procedures."
Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.
| Bile Esculin Agar | |||||
| Approximate Formula* Per Liter Purified Water | |||||
| Pancreatic Digest of Gelatin | 5.0 | g | |||
| Beef Extract | 3.0 | g | |||
| Oxgall | 20.0 | g | |||
| Ferric Citrate | 0.5 | g | |||
| Esculin | 1.0 | g | |||
| Agar | 14.0 | g | |||
| *Adjusted and/or supplemented as required to meet performance criteria. | |||||
For in vitro Diagnostic Use.
If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.
Observe aseptic techniques and established precautions against microbiological hazards throughout all procedures. After use, prepared plates, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.
Information shown on this page is a short summary extracted from the QC/PI Manual, available as a PDF under the Related Documents section of this page.
