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CDC Anaerobe Laked Sheep Blood Agar with Kanamycin and Vancomycin is used for the selective isolation of fastidious and slow-growing, obligately anaerobic gram-negative bacteria from a variety of clinical and nonclinical materials.

The isolation of obligately anaerobic bacteria from clinical and nonclinical materials requires the use of selective, nonselective and enrichment media.¹ The choice of media to be employed is based upon the type of material and the results of direct microscopic observation. Nonselective media are used to isolate organisms present in low numbers and to provide an indication of the numbers and types of organisms present in the sample. Selective media are employed to facilitate recovery of the desired organisms present in mixed populations.

CDC Anaerobe Laked Sheep Blood Agar with Kanamycin and Vancomycin was formulated by Dowell et al. of the Centers for Disease Control and Prevention as an enriched, selective medium for the isolation and cultivation of Prevotella melaninogenica, Fusobacterium necrophorum, Fusobacterium nucleatum and other fastidious, obligately anaerobic, gram-negative bacilli, from clinical materials containing mixed populations.^2,3 The medium employs BBL Trypticase Soy Agar supplemented with additional agar, yeast extract, vitamin K₁, hemin, cystine, 5% sheep blood, kanamycin and vancomycin. The combination of kanamycin and vancomycin for use in selective isolation of gram-negative anaerobes was first described by Finegold et al.⁴ Vancomycin, however, may inhibit Porphyromonas asaccharolytica.⁵ This medium is similar to CDC Anaerobe 5% Sheep Blood Agar with Kanamycin and Vancomycin except that the blood has been laked, by subjecting it to three freeze-thaw cycles, for improved pigmentation of the P. melaninogenica-P. asaccharolytica group.⁴

See "Quality Control Procedures."

Quality Control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.

For in vitro Diagnostic Use.

If excessive moisture is observed, invert bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.

Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"^11-14 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared plates, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.