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Lecithin Lactose Agar
| Cat. # | Description | Qty. | Unit |
| 221858 | Lecithin Lactose Agar | 10 | SP |
Lecithin Lactose Agar is recommended for the isolation and differentiation of histotoxic clostridia from clinical specimens.1 It is particularly useful in differentiating Clostridium perfringens, C. sordellii, C. novyi, C. septicum and C. histolyticum.
| Catalog # | Description | Quantity | Unit |
| 221858 | Lecithin Lactose Agar | 10 | SP |
BBL™ Lecithin Lactose Agar
Revision: 06 Revision Date: 10/01/2006
Lecithin Lactose Agar is recommended for the isolation and differentiation of histotoxic clostridia from clinical specimens.1 It is particularly useful in differentiating Clostridium perfringens, C. sordellii, C. novyi, C. septicum and C. histolyticum.
Culture media containing egg yolks were useful in isolating, cultivating and identifying species of histotoxic clostridia. In 1948, McClung and Toabe reported on the use of an egg yolk medium for this purpose.1 Willis and Hobbs added milk and lactose to the egg yolk in a formulation designed to group clostridia on the basis of production of lecithinase, hydrolysis of casein and lactose fermentation.2 Neomycin sulfate was included in their formulation in order to make it a selective medium.
Willis, in response to problems in the obtaining and processing of antibiotic-free eggs, developed an egg-free medium in which purified lecithin was substituted for the egg yolk.3 In order to further refine the growth-promoting and selective properties, Ellner and O'Donnell developed the formulation, designated as Lecithin Lactose Agar in which a decreased concentration of neomycin was employed and to which sodium azide was added.4 This medium continues to be an important one for visualizing and characterizing the histotoxic clostridia.
See "Quality Control Procedures."
Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory’s standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.
| Lecithin Lactose Agar | |||||
| Approximate Formula* Per Liter Purified Water | |||||
| Pancreatic Digest of Casein | 12.65 | g | |||
| Peptic Digest of Animal Tissue | 5.5 | g | |||
| Pancreatic Digest of Heart Muscle | 3.3 | g | |||
| Yeast Extract | 3.85 | g | |||
| Corn Starch | 1.1 | g | |||
| Sodium Chloride | 5.5 | g | |||
| Agar | 15.0 | g | |||
| Lactose | 10.0 | g | |||
| Sodium Azide | 0.2 | g | |||
| Neomycin Sulfate | 0.15 | g | |||
| L-Cysteine Hydrochloride | 0.5 | g | |||
| Calcium Chloride | 0.05 | g | |||
| Egg Lecithin | 0.66 | g | |||
| Bromcresol Purple | 25.0 | mg | |||
| *Adjusted and/or supplemented as required to meet performance criteria. | |||||
For in vitro Diagnostic Use.
If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.
Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"5-8 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared plates, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.
Information shown on this page is a short summary extracted from the QC/PI Manual, available as a PDF under the Related Documents section of this page.
