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Sabouraud Dextrose Agar
Cat. # Desc. Qty. Unit
221988 Sabouraud Dextrose Agar 10 SP
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Intended Use:

Sabouraud Dextrose Agar, Sterile Pack environmental sampling plates are used for the detection and enumeration of microorganisms present on surfaces of sanitary importance. The Sterile Pack plates are particularly useful for monitoring surfaces in clean rooms and other environmentally-controlled areas.

The contact-style plates are also recommended for use in air sampling equipment such as the Surface Air System.

Catalog # Description Quantity Unit
221988 Sabouraud Dextrose Agar 10 SP

BBL™ Prepared Sterile Pack Plates for Environmental Monitoring Sabouraud Dextrose Agar, Sterile Pack
Revision: 0200    Revision Date: 02/01/2000   

Intended Use: «

Sabouraud Dextrose Agar, Sterile Pack environmental sampling plates are used for the detection and enumeration of microorganisms present on surfaces of sanitary importance. The Sterile Pack plates are particularly useful for monitoring surfaces in clean rooms and other environmentally-controlled areas.

The contact-style plates are also recommended for use in air sampling equipment such as the Surface Air System.

Intended Use: » Intended Use:

Sabouraud Dextrose Agar, Sterile Pack environmental sampling plates are used for the detection and enumeration of microorganisms present on surfaces of sanitary importance. The Sterile Pack plates are particularly useful for monitoring surfaces in clean rooms and other environmentally-controlled areas.

The contact-style plates are also recommended for use in air sampling equipment such as the Surface Air System.


Product Summary: «

Environmental sampling plates are specially constructed so that an agar medium can be over-filled, producing a meniscus or dome-shaped surface that can be pressed onto a surface for sampling its microbial burden. These plates are used in a variety of programs to establish and monitor cleaning techniques and schedules.1-5

After touching the surface to be sampled with the medium, the dish is covered and incubated at an appropriate temperature. The presence and number of microorganisms is determined by the appearance of colonies on the surface of the agar medium.6 Collection of samples from the same area before and after cleaning and treatment with a disinfectant permits the evaluation of the efficacy of sanitary procedures.

Sabouraud Dextrose Agar was devised by Sabouraud for the cultivation of dermatophytes.7 The low pH of approximately 5.6 is favorable for the growth of fungi, especially dermatophytes, and inhibitory to contaminating bacteria in clinical specimens.8-10

Product Summary: » Product Summary:

Environmental sampling plates are specially constructed so that an agar medium can be over-filled, producing a meniscus or dome-shaped surface that can be pressed onto a surface for sampling its microbial burden. These plates are used in a variety of programs to establish and monitor cleaning techniques and schedules.1-5

After touching the surface to be sampled with the medium, the dish is covered and incubated at an appropriate temperature. The presence and number of microorganisms is determined by the appearance of colonies on the surface of the agar medium.6 Collection of samples from the same area before and after cleaning and treatment with a disinfectant permits the evaluation of the efficacy of sanitary procedures.

Sabouraud Dextrose Agar was devised by Sabouraud for the cultivation of dermatophytes.7 The low pH of approximately 5.6 is favorable for the growth of fungi, especially dermatophytes, and inhibitory to contaminating bacteria in clinical specimens.8-10


User Quality Control: « User Quality Control:

1. Examine the plates for signs of deterioration as described under "Product Deterioration."

2. Check performance by inoculating a representative sample of plates with pure cultures of stable control organisms that give known, desired reactions. The following test strains are recommended:

TEST STRAIN EXPECTED RESULTS
Candida albicans
ATCC™ 60193

Trichophyton mentagrophytes
ATCC 9533

Aspergillus fumigatus
ATCC 26934
Growth


Growth


Growth

Reagents: « Reagents:

Formula:

Sabouraud Dextrose Agar
  Approximate Formula* Per Liter Purified Water
  Pancreatic Digest of Casein  5.0   g
  Peptic Digest of Animal Tissue  5.0  
  Dextrose  40.0  
  Agar  15.0  
  * Adjusted and/or supplemented as required to meet performance criteria and additionally, to compensate for radiation effects.

Warnings and Precautions: « Warnings and Precautions:

For Laboratory Use

Observe aseptic techniques and established precautions against microbiological hazards throughout all procedures. After use, prepared plates, specimen containers and other contaminated materials should be sterilized by autoclaving.



Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.