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Listeria O Antigen Type 1 (Slide), 5 mL
Cat. # Desc. Qty. Unit
223031 Listeria O Antigen Type 1 (Slide), 5 mL 1 EA
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Intended Use:

Difco™ Listeria O Antisera Types 1, 4 and Poly are used for identifying Listeria monocytogenes in the slide and tube agglutination tests.

Difco Listeria O Antigens Types 1 and 4 (Slide and Tube) are used as positive controls in the slide and tube agglutination tests, respectively.

Catalog # Description Quantity Unit
223031 Listeria O Antigen Type 1 (Slide), 5 mL
Quality control of rapid slide test using Listeria O Antiserum Type 1 (223001)
1 EA

Difco™ Listeria Antisera and Antigens
Revision: 0105    Revision Date: 01/01/2005   

Intended Use: «

Difco™ Listeria O Antisera Types 1, 4 and Poly are used for identifying Listeria monocytogenes in the slide and tube agglutination tests.

Difco Listeria O Antigens Types 1 and 4 (Slide and Tube) are used as positive controls in the slide and tube agglutination tests, respectively.

Intended Use: » Intended Use:

Difco™ Listeria O Antisera Types 1, 4 and Poly are used for identifying Listeria monocytogenes in the slide and tube agglutination tests.

Difco Listeria O Antigens Types 1 and 4 (Slide and Tube) are used as positive controls in the slide and tube agglutination tests, respectively.


Product Summary: «

First described in 1926 by Murray, Webb and Swann,1 Listeria monocytogenes is a widespread problem in public health and the food industries. This organism can cause human illness and death, particularly in immunocompromised individuals and pregnant women.2 The first reported foodborne outbreak of listeriosis was in 19853 and, since then, microbiological and epidemiological evidence from both sporadic and epidemic cases of listeriosis has shown that the principal route of transmission is via the consumption of foodstuffs contaminated with L. monocytogenes.4

The organism has been isolated from commercial dairy and other food processing plants and is ubiquitous in nature, being present in a wide-range of unprocessed foods and in soil, sewage, silage and river water.5

Listeria spp. grow over a pH range of 4.4-9.6 and survive in food products with pH levels outside these parameters.6 Listeria spp. are microaerophilic, gram-positive, asporogenous, non-encapsulated, non-branching, regular, short, motile rods. Motility is most pronounced at 20°C.

Strains of Listeria spp. are divided into serotypes based on cellular (O) and flagellar (H) antigens.7 Thirteen serotypes of L. monocytogenes are known. Most human disease is caused by serotypes 1/2a, 1/2b and 4b.8

Product Summary: » Product Summary:

First described in 1926 by Murray, Webb and Swann,1 Listeria monocytogenes is a widespread problem in public health and the food industries. This organism can cause human illness and death, particularly in immunocompromised individuals and pregnant women.2 The first reported foodborne outbreak of listeriosis was in 19853 and, since then, microbiological and epidemiological evidence from both sporadic and epidemic cases of listeriosis has shown that the principal route of transmission is via the consumption of foodstuffs contaminated with L. monocytogenes.4

The organism has been isolated from commercial dairy and other food processing plants and is ubiquitous in nature, being present in a wide-range of unprocessed foods and in soil, sewage, silage and river water.5

Listeria spp. grow over a pH range of 4.4-9.6 and survive in food products with pH levels outside these parameters.6 Listeria spp. are microaerophilic, gram-positive, asporogenous, non-encapsulated, non-branching, regular, short, motile rods. Motility is most pronounced at 20°C.

Strains of Listeria spp. are divided into serotypes based on cellular (O) and flagellar (H) antigens.7 Thirteen serotypes of L. monocytogenes are known. Most human disease is caused by serotypes 1/2a, 1/2b and 4b.8


User Quality Control: « User Quality Control:

At the time of use, test both positive and negative antigen controls to check performance of the antiserum, techniques and methodology. If results of the positive control or negative control are not as described, the test is invalid and results cannot be reported.

Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent NCCLS guidance and CLIA regulations for appropriate Quality Control practices.


Reagents: « Reagents:

Difco Listeria O Antisera Types 1, 4 and Poly are lyophilized, polyclonal rabbit antisera containing approximately 0.04% Thimerosal as a preservative. Listeria O Antisera Types 1 and 4 are specific for the respective serotypes of L. monocytogenes while Listeria O Antiserum Poly contains agglutinins for L. monocytogenes serotypes 1 and 4.

Difco Listeria O Antigens Types 1 and 4 (Tube) and (Slide) are suspensions of appropriate L. monocytogenes serotypes containing 0.3% formaldehyde as a preservative. When used according to the suggested procedure, the reagents will yield the following:

Reagent Vial Number of tests
Listeria O Antiserum 1 mL 10 tube tests or 400 slide tests
Listeria O Antigen (Slide) 5 mL 100 slide tests
Listeria O Antigen (Tube) 25 mL 5 tube tests

Warnings and Precautions: « Warnings and Precautions:

For in vitro Diagnostic Use.

The Packaging of This Product Contains Dry Natural Rubber.

Observe aseptic techniques and established precautions against microbiological hazards throughout all procedures. After use, specimens, containers, slides, tubes and other contaminated material must be sterilized by autoclaving. Directions for use should be followed carefully.



Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.