United States
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 Vibrio Cholerae Antiserum Poly, 3 mL |
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| Cat. # | Desc. | Qty. | Unit |
| 224321 | Vibrio Cholerae Antiserum Poly, 3 mL | 1 | EA |
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Difco™ Vibrio Cholerae antisera are recommended for use in slide agglutination tests for the identification and serotyping of Vibrio cholerae.
| Catalog # | Description | Quantity | Unit |
| 224321 |
Vibrio Cholerae Antiserum Poly, 3 mL
Rabbit serum for slide agglutination test Contains agglutinins for V. cholerae, inaba and ogawa.
Contains Mercury. Dispose of Properly. |
1 | EA |
Difco™ Vibrio Cholerae antisera are recommended for use in slide agglutination tests for the identification and serotyping of Vibrio cholerae. Difco™ Vibrio Cholerae antisera are recommended for use in slide agglutination tests for the identification and serotyping of Vibrio cholerae. V. cholerae is the causative agent of cholera. The spread of the disease is primarily through contaminated water and the fecal-oral route. If not treated, patients with severe cholera may die within five hours from massive fluid and electrolyte loss.1 Because Vibrio spp. are natural inhabitants of aquatic environments, food products, such as uncooked seafoods or seafoods incorrectly handled, can spread infection. Gastrointestinal symptoms of cholera include "rice water stools" caused by a potent enterotoxin. From humans, the primary specimen is feces. Seafood products are frequently tested as vehicles of human infection. Gardner and Ventkatraman2 defined the identification of Vibrio cholerae as the recognition of the somatic antigen O1 (O group 1) as evidenced by agglutination with O1 (polyvalent) antiserum. V. cholerae of the O1 serogroup are divided into serotypes: Ogawa, Inaba and Hikojima.1,3 The antigenic factors are:
V. cholerae are facultative anaerobes that by microscopic morphology are gram-negative curved or straight bacilli. The microorganisms either require sodium chloride or grow best in its presence. Various special media designed to select and cultivate this microorganism are used in clinical and food microbiology laboratories and for environmental testing. V. cholerae is the causative agent of cholera. The spread of the disease is primarily through contaminated water and the fecal-oral route. If not treated, patients with severe cholera may die within five hours from massive fluid and electrolyte loss.1 Because Vibrio spp. are natural inhabitants of aquatic environments, food products, such as uncooked seafoods or seafoods incorrectly handled, can spread infection. Gastrointestinal symptoms of cholera include "rice water stools" caused by a potent enterotoxin. From humans, the primary specimen is feces. Seafood products are frequently tested as vehicles of human infection. Gardner and Ventkatraman2 defined the identification of Vibrio cholerae as the recognition of the somatic antigen O1 (O group 1) as evidenced by agglutination with O1 (polyvalent) antiserum. V. cholerae of the O1 serogroup are divided into serotypes: Ogawa, Inaba and Hikojima.1,3 The antigenic factors are:
V. cholerae are facultative anaerobes that by microscopic morphology are gram-negative curved or straight bacilli. The microorganisms either require sodium chloride or grow best in its presence. Various special media designed to select and cultivate this microorganism are used in clinical and food microbiology laboratories and for environmental testing. At the time of use, test both positive and negative antigen controls to check performance of the antisera, techniques and methodology. Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent NCCLS guidance and CLIA regulations for appropriate Quality Control practices. Difco Vibrio Cholerae antisera are stable, lyophilized polyclonal rabbit Vibrio cholerae O1 antisera containing approximately 0.04% Thimerosal as a preservative. Difco Vibrio Cholerae Antiserum Ogawa and Difco Vibrio Cholerae Antiserum Inaba are monospecific absorbed antisera. Difco Vibrio Cholerae Antiserum Poly and the absorbed Difco Vibrio Cholerae Antiserum Ogawa and Difco Vibrio Cholerae Antiserum Inaba contain the following antibodies:
When reconstituted and used as described, each vial of Difco Vibrio Cholerae antisera contains sufficient reagent for 20 slide tests. For in vitro Diagnostic Use. The Packaging of This Product Contains Dry Natural Rubber. Observe aseptic techniques and established precautions against microbiological hazards throughout all procedures. After use, specimens, containers, slides, tubes and other contaminated material must be sterilized by autoclaving. Directions for use should be followed carefully. | |||||||||||||||||||||||||||||||||||||||||
Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.
