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TTC Solution 1% (Triphenyltetrazolium Chloride) is ready for use in the preparation of culture media.

TTC Solution 1% is prepared from microbiologically tested 2, 3, 5 triphenyltetrazolium chloride (TTC) for use in all microbiological methods utilizing triphenyltetrazolium chloride. It is used as a redox indicator in culture media for differentiating bacteria.

Chapman¹ reported that the addition of TTC to Tergitol™ 7 Agar permitted the detection and confirmation of E. coli after only 10 h incubation. Chapman also reported that Tergitol 7 Agar with added TTC gave a selective medium for the detection and isolation of Candida albicans. Chapman² added TTC, Tergitol 7 and Brom Cresol Purple to Sabouraud Maltose Agar for the isolation and identification of C. albicans and other fungi. Pagano, Levin and Trejo³ incorporated TTC in Pagano Levin Base for the detection and isolation of Candida species.

Slanetz and Bartley⁴ used TTC in m-Enterococcus Agar for the enumeration of enterococci. On this medium the colonies appear as pink to dark maroon in color. Kenner, Clark and Kabler⁵ used TTC with KF Streptococcus Agar and Broth to detect and enumerate streptococci in surface waters.

For Laboratory Use