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BBL™ DrySlide™ Oxidase



Cat. # Description Qty. Unit
231746 BBL™ DrySlide™ Oxidase
75 SP
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Intended Use:

BBL™ DrySlide™ Oxidase is used for determining the oxidase reaction of bacteria.


Catalog # Description Quantity Unit
231746 BBL™ DrySlide™ Oxidase
75 SP

BD BBL™ DrySlide™ Oxidase
Revision: 2010/06     Revision Date: 06/01/2010    

Intended Use:

BBL™ DrySlide™ Oxidase is used for determining the oxidase reaction of bacteria.



Product Summary:

The oxidase test is a qualitative procedure for determining the presence or absence of cytochrome c oxidase activity in bacteria. This activity is dependent on the presence of an intracellular cytochrome oxidase system that catalyzes the oxidation of cytochrome c by molecular oxygen, which then serves as the terminal electron acceptor in the organism's electron transport system.1

Organisms containing cytochrome c as part of their respiratory chain are oxidase positive and turn the reagent purple; organisms lacking cytochrome c as part of their respiratory chain do not oxidize the reagent, leaving it colorless within the time limits of the test, and are oxidase negative.



User Quality Control:

Identity Specifications — 2" x 2" slides with four reaction areas. The slides are contained in foil pouches. Reaction area should be white paper with no blue tint. Each pouch contains 3 slides.

Performance Response — Perform the oxidase test following the above procedure with the test organisms listed below.

Organism ATCC™ Oxidase Reaction Color Response
Escherichia coli 25922 Colorless
(no change) to gray
Neisseria sicca 9913 + Dark purple
Pseudomonas aeruginosa 27853 + Dark purple

Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent NCCLS guidance and CLIA regulations for appropriate Quality Control practices.



Reagents:

BBL DrySlide Oxidase is a disposable slide having four filter paper reaction areas containing N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride and ascorbic acid as a reducing agent and stabilizer.



Warnings and Precautions:

For in vitro Diagnostic Use.

Handle BBL DrySlide Oxidase only by the slide mount. Do not touch the reaction area.

Follow established laboratory procedures in handling and disposing of infectious materials.



Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.