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BBL™ DrySlide™ PYR Kit, containing BBL™ DrySlide™ PYR and BBL™ DrySlide™ PYR Color Developer, is used for detecting hydrolysis of PYR in the rapid presumptive identification of group A streptococci and group D enterococci from pure culture.

BBL DrySlide PYR, provided in an easy-to-handle slide format, and BBL DrySlide PYR Color Developer, provided in a reagent dispenser, accommodate up to four tests per slide/dispenser.

The PYR test is a qualitative procedure for determining the ability of streptococci to enzymatically hydrolyze L-pyrrolidonyl-β-naphthylamide (PYR). Facklam, Thacker, Fox and Eriquez¹ reported that 98% of group A streptococci and 96% of group D enterococci hydrolyze PYR. Although Aerococcus species are rarely isolated in the clinical laboratory,² these organisms are also expected to hydrolyze PYR.³

Facklam et al. further reported that 98% of group B streptococci, 100% of non-group A, B and D streptococci, 100% of group D non-enterococci and 82% of viridans streptococci yield negative PYR test results.¹

1. Examine BBL DrySlide PYR.

2. Before use, the dry reaction area should be white in color. After wetting, the reaction area should remain white. After dispensing BBL DrySlide PYR Color Developer, the reaction area becomes pale yellow.

3. Check product performance by testing with positive and negative control cultures, such as those listed below.

Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent NCCLS guidance and CLIA regulations for appropriate Quality Control practices.

BBL DrySlide PYR is a disposable 2" by 2" slide having four filter paper reaction areas containing L-pyrrolidonyl-β-naphthylamide.

BBL DrySlide PYR Color Developer is 0.015% p-dimethylaminocinnamaldehyde prepared according to a conventional formulation.

For in vitro Diagnostic Use.

Handle BBL DrySlide PYR only by the slide mount; do not touch the reaction area.

Use aseptic technique and established laboratory procedures in handling and disposing of infectious materials.