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Tinsdale Enrichment, Desiccated, 15 mL
Cat. # Desc. Qty. Unit
234210 Tinsdale Enrichment, Desiccated, 15 mL 6 SP
Intended Use:

Difco Tinsdale Enrichment Desiccated is used with Difco Tinsdale Agar Base for primary isolation and differentiation of Corynebacterium diphtheriae.

Catalog # Description Brand Quantity Unit
234210 Tinsdale Enrichment, Desiccated, 15 mL
Enrichment used with Tinsdale Base for the differential isolation of C. diphtheriae
 Difco 6 SP

Difco™ Tinsdale Enrichment Desiccated
Revision: 0104    Revision Date: 01/01/2004   
Intended Use: «

Difco Tinsdale Enrichment Desiccated is used with Difco Tinsdale Agar Base for primary isolation and differentiation of Corynebacterium diphtheriae.

Intended Use: » Intended Use:

Difco Tinsdale Enrichment Desiccated is used with Difco Tinsdale Agar Base for primary isolation and differentiation of Corynebacterium diphtheriae.

Product Summary: «

Tinsdale Agar Base, supplemented with Tinsdale Enrichment, is employed in the cultural diagnosis of diphtheria. Diphtheria, an acute infectious disease primarily of the upper respiratory tract but occasionally of the skin,1 is caused by toxigenic strains of Corynebacterium diphtheriae. The three biotypes are mitis, intermedius and gravis.1 The signs and symptoms of the disease are a pharyngeal membrane, sore throat, malaise, headache and nausea.2 Death can result from respiratory obstruction by the membrane or myocarditis caused by the toxin.2

Tinsdale3 developed a serum-cystine-thiosulfite-tellurite agar medium for the primary isolation and differentiation of C. diphtheriae. This formulation distinguished between C. diphtheriae and diphtheroids which exhibited similar characteristics. The differential principle is based on the capacity of C. diphtheriae to produce a brown or black halo around the colonies.

Billings4 simplified Tinsdale Basal Medium by using Proteose Peptone No. 3 as a nutrient source. This modification improved the differential qualities and recovery of C. diphtheriae. Moore and Parsons5 confirmed the halo formation of C. diphtheriae with one exception; C. ulcerans occasionally produced colonies similar to C. diphtheriae and required biochemical identification.

Product Summary: » Product Summary:

Tinsdale Agar Base, supplemented with Tinsdale Enrichment, is employed in the cultural diagnosis of diphtheria. Diphtheria, an acute infectious disease primarily of the upper respiratory tract but occasionally of the skin,1 is caused by toxigenic strains of Corynebacterium diphtheriae. The three biotypes are mitis, intermedius and gravis.1 The signs and symptoms of the disease are a pharyngeal membrane, sore throat, malaise, headache and nausea.2 Death can result from respiratory obstruction by the membrane or myocarditis caused by the toxin.2

Tinsdale3 developed a serum-cystine-thiosulfite-tellurite agar medium for the primary isolation and differentiation of C. diphtheriae. This formulation distinguished between C. diphtheriae and diphtheroids which exhibited similar characteristics. The differential principle is based on the capacity of C. diphtheriae to produce a brown or black halo around the colonies.

Billings4 simplified Tinsdale Basal Medium by using Proteose Peptone No. 3 as a nutrient source. This modification improved the differential qualities and recovery of C. diphtheriae. Moore and Parsons5 confirmed the halo formation of C. diphtheriae with one exception; C. ulcerans occasionally produced colonies similar to C. diphtheriae and required biochemical identification.

User Quality Control: « User Quality Control:

Prepare Tinsdale Agar per label directions. Inoculate to obtain discrete colonies and stab several times using an inoculating needle; incubate at 35 ± 2șC for 18–48 h.

ORGANISM ATCC™ INOCULUM CFU RECOVERY APPEARANCE
Corynebacterium diphtheriae biotype gravis 8028 102–103 Good Brown with halos
Corynebacterium diphtheriae biotyope mitis 8024 102–103 Good Brown with halos
Klebsiella pneumoniae 13883 102–103 Marked to complete inhibition –
Streptococcus pyogenes 19615 102–103 Poor to fair Brown to black without halos


Reagents: « Reagents:
Difco™ Tinsdale Enrichment Desiccated
  Approximate Formula* Per Liter Purified Water
  Bovine Serum  333   mL
  Horse Serum  380   mL
  L-Cystine  2.0   g
  Potassium Tellurite  1.4   g
  Sodium Thiosulfate  2.8   g
  * Adjusted and/or supplemented as required to meet performance criteria.


Warnings and Precautions: « Warnings and Precautions:

For Laboratory Use.

Observe aseptic techniques and established precautions against microbiological hazards throughout all procedures. After use, prepared plates, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.



Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.