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BBL™ Crystal™ Enteric/Nonfermenter ID Kit

Cat. #	Description	Qty.	Unit
245000	BBL™ Crystal™ Enteric/Nonfermenter ID Kit Intended for the identification of clinically significant aerobic gram-negative bacteria that belong to the family Enterobacteriaceae as well as some of the more frequently isolated glucose nonfermenting gram-negative bacilli of human origin	20	SP

Intended Use:

The BBL Crystal™ Enteric/Nonfermenter (E/NF) Identification (ID) System is for the identification of aerobic gram-negative bacteria that belong to the family Enterobacteriaceae as well as some of the more frequently isolated glucose fermenting and nonfermenting gram-negative bacilli.

Catalog #	Description	Quantity	Unit
245000	BBL™ Crystal™ Enteric/Nonfermenter ID Kit Intended for the identification of clinically significant aerobic gram-negative bacteria that belong to the family Enterobacteriaceae as well as some of the more frequently isolated glucose nonfermenting gram-negative bacilli of human origin	20	SP

BBL Crystal™ Identification Systems
Enteric/Nonfermenter ID Kit
Revision: 2010/10 Revision Date: 10/01/2010

Intended Use:

Product Summary:

The BBL Crystal E/NF ID System is a miniaturized identification method. Many of the tests used are modifications of classical methods. These include tests for fermentation, oxidation, degradation and hydrolysis of various substrates. In addition, there are chromogen linked substrates to detect enzymes that microbes use to metabolize various substrates.^1-5

The BBL Crystal E/NF ID kit is comprised of (i) BBL Crystal E/NF panel lids, (ii) BBL Crystal bases and (iii) BBL Crystal™ Enteric/Stool ID Inoculum Fluid (IF) tubes. The lid contains 30 dehydrated substrates on tips of plastic prongs. The base has 30 reaction wells. Test inoculum is prepared with the inoculum fluid and is used to fill all 30 wells in the base. When the lid is aligned with the base and snapped in place, the test inoculum rehydrates the dried substrates and initiates test reactions.

Following an incubation period, the wells are examined for color changes. Color changes result from metabolic activities of the microorganisms. The resulting pattern of the 30 reactions is converted into a ten digit profile number that is used as the basis for identification.⁶ Biochemical and enzymatic reaction patterns for the 30 BBL Crystal E/NF ID substrates with a wide variety of microorganisms are stored in the BBL Crystal E/NF ID database. Identification is derived from a comparative analysis of the reaction pattern of the test isolate to those held in the database. A complete list of taxa that comprises the current E/NF database is provided in Table 1 (page 36).

User Quality Control:

Quality control testing is recommended for each lot of panels as follows –

1. Set up a BBL Crystal E/NF panel with Klebsiella pneumoniae ATCC™ 33495 per recommended procedure (refer to "Test Procedure").

2. Incubate panel for 18 – 20 h at 35 – 37°C.

3. Read panel with BBL Crystal Light Box or Panel Viewer and BBL Crystal E/NF Color Reaction Chart; record reactions using the BBL Crystal E/NF Report Pad. Alternatively, read the panel on the BBL Crystal AutoReader.

4. Compare recorded reactions with those listed in Table 2 (page 37). If discrepant results are obtained, confirm purity of quality control strain before contacting BD Technical Services.

Expected test results for additional quality control test strains are also listed in Table 2 (page 37).

Reagents:

The BBLCrystal E/NF ID panel contains 30 enzymatic and biochemical substrates as described below. Panel location indicates the row and column where the well is located (example: 1J refers to Row 1 in Column J).

Reagents and Principles of Tests Employed in the BBLCrystal E/NF ID System

Panel Location	Active Ingredient	Code	Approx. Amt. (g/10 mL)	Pos.	Neg.	Principle (Reference)

4A	Arabinose	ARA	3.5	Gold/Yellow	Orange/Red	Utilization of carbohydrate results in lower pH and change in indicator (Phenol red).^7-10

4B	Mannose	MNS	3.0	Gold/Yellow	Orange/Red

4C	Sucrose	SUC	2.8	Gold/Yellow	Orange/Red

4D	Melibiose	MEL	1.0	Gold/Yellow	Orange/Red

4E	Rhamnose	RHA	3.0	Gold/Yellow	Orange/Red

4F	Sorbitol	SOR	3.5	Gold/Yellow	Orange/Red

4G	Mannitol	MNT	1.8	Gold/Yellow	Orange/Red

4H	Adonitol	ADO	2.5	Gold/Yellow	Orange/Red

4I	Galactose	GAL	1.5	Gold/Yellow	Orange/Red

4J	Inositol	INO	1.3	Gold/Yellow	Orange/Red

2A	p-n-p-phosphate	PHO	0.025	Yellow	Colorless	Enzymatic hydrolysis of the colorless aryl substituted glycoside or phospate ester releases yellow p-nitrophenol.^1-5

2B	p-n-p α-β-glucoside	BGL	0.025	Yellow	Colorless

2C	p-n-p-β-galactoside	NPG	0.06	Yellow	Colorless

2D	Proline nitroanilide	PRO	0.07	Yellow	Colorless	Enzymatic hydrolysis of colorless amide substrate releases yellow p-nitroaniline.^1-5

2E	p-n-p bis-phosphate	BPH	0.02	Yellow	Colorless	Enzymatic hydrolysis of the colorless aryl substituted glycoside or phospate ester releases yellow p-nitrophenol.^1-5

2F	p-n-p-xyloside	BXY	0.03	Yellow	Colorless

2G	p-n-p-α-arabinoside	AAR	0.03	Yellow	Colorless

2H	p-n-p-phosphorylcholine	PHC	0.03	Yellow	Colorless

2I	p-n-p-β-glucuronide	GLR	0.02	Yellow	Colorless

2J	p-n-p-N-acetyl glucosaminide	NAG	0.04	Yellow	Colorless

1A	γ-L-glutamyl p-nitroanilide	GGL	0.03	Yellow	Colorless	Enzymatic hydrolysis of the colorless amide substrate releases yellow p-nitroaniline.^1-5

1B	Esculin	ESC	0.14	Brown/Maroon	Clear/Straw	Hydrolysis of esculin results in a black precipitate in the presence of ferric ion.¹¹

1C	p-nitro-DL-phenylalanine	PHE	0.1	Gold/Dk. Orange	Yellow	Oxidative deamination of phenylalanine results in a brown color in the presence of ferric ion.^7,11

1D	Urea	URE	0.2	Aqua/Blue	Yellow/Green	Hydrolysis of urea and the resulting ammonia change the pH indicator color (Bromthymol blue).^7,11,12

1E	Glycine	GLY	0.7	Aqua/Blue	Yellow/Green	Degradation of glycine results in alkaline metabolites that change color of the pH indicator (Bromthymol blue).¹³

1F	Citrate	CIT	0.8	Aqua/Blue	Yellow/Green	Utilization of citrate results in alkaline metabolites that change color of the pH indicator (Bromthymol blue).^7,14

1G	Malonic acid	MLO	1.5	Aqua/Blue	Yellow/Green	Utilization of malonate results in alkaline metabolites that change color of the pH indicator (Bromthymol blue).¹¹

1H	Triphenyl Tetrazolium chloride	TTC	0.15	Pink/Red*	Clear	Reduction of the tetrazolium compound results in formation of a red formazan.¹³

1I	Arginine	ARG	1.5	Red/Purple	Yellow/Brown	Anaerobic catabolism results in pH rise and change in the color of the indicator (Bromcresol purple).^7,15

1J	Lysine	LYS	0.5	Red/Purple	Yellow/Brown

*Precipitate may or may not be visible.

After use, all infectious materials including plates, cotton swabs, inoculum tubes, filter papers used for oxidase or indole tests and BBL Crystal panels must be autoclaved prior to disposal or incinerated.

Warnings and Precautions:

For in vitro Diagnostic Use

Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.

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