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BBL™ Crystal™ Enteric/Nonfermenter ID Kit
Cat. # Desc. Qty. Unit
245000 BBL™ Crystal™ Enteric/Nonfermenter ID Kit 20 SP
Intended Use:

The BBL Crystal™ Enteric/Nonfermenter (E/NF) Identification (ID) System is for the identification of aerobic gram-negative bacteria that belong to the family Enterobacteriaceae as well as some of the more frequently isolated glucose fermenting and nonfermenting gram-negative bacilli.

Catalog # Description Quantity Unit
245000 BBL™ Crystal™ Enteric/Nonfermenter ID Kit
Intended for the identification of clinically significant aerobic gram-negative bacteria that belong to the family Enterobacteriaceae as well as some of the more frequently isolated glucose nonfermenting gram-negative bacilli of human origin
 20 SP

BBL Crystal™ Identification Systems
Enteric/Nonfermenter ID Kit
Revision: 0607    Revision Date: 06/01/2007   
Intended Use: «

The BBL Crystal™ Enteric/Nonfermenter (E/NF) Identification (ID) System is for the identification of aerobic gram-negative bacteria that belong to the family Enterobacteriaceae as well as some of the more frequently isolated glucose fermenting and nonfermenting gram-negative bacilli.

Intended Use: » Intended Use:

The BBL Crystal™ Enteric/Nonfermenter (E/NF) Identification (ID) System is for the identification of aerobic gram-negative bacteria that belong to the family Enterobacteriaceae as well as some of the more frequently isolated glucose fermenting and nonfermenting gram-negative bacilli.

Product Summary: «

The BBL Crystal E/NF ID System is a miniaturized identification method. Many of the tests used are modifications of classical methods. These include tests for fermentation, oxidation, degradation and hydrolysis of various substrates. In addition, there are chromogen linked substrates to detect enzymes that microbes use to metabolize various substrates.1-5

The BBL Crystal E/NF ID kit is comprised of (i) BBL Crystal E/NF panel lids, (ii) BBL Crystal bases and (iii) BBL Crystal™ Enteric/Stool ID Inoculum Fluid (IF) tubes. The lid contains 30 dehydrated substrates on tips of plastic prongs. The base has 30 reaction wells. Test inoculum is prepared with the inoculum fluid and is used to fill all 30 wells in the base. When the lid is aligned with the base and snapped in place, the test inoculum rehydrates the dried substrates and initiates test reactions.

Following an incubation period, the wells are examined for color changes. Color changes result from metabolic activities of the microorganisms. The resulting pattern of the 30 reactions is converted into a ten digit profile number that is used as the basis for identification.6 Biochemical and enzymatic reaction patterns for the 30 BBL Crystal E/NF ID substrates with a wide variety of microorganisms are stored in the BBL Crystal E/NF ID database. Identification is derived from a comparative analysis of the reaction pattern of the test isolate to those held in the database. A complete list of taxa that comprises the current E/NF database is provided in Table 1 (page 36).

Product Summary: » Product Summary:

The BBL Crystal E/NF ID System is a miniaturized identification method. Many of the tests used are modifications of classical methods. These include tests for fermentation, oxidation, degradation and hydrolysis of various substrates. In addition, there are chromogen linked substrates to detect enzymes that microbes use to metabolize various substrates.1-5

The BBL Crystal E/NF ID kit is comprised of (i) BBL Crystal E/NF panel lids, (ii) BBL Crystal bases and (iii) BBL Crystal™ Enteric/Stool ID Inoculum Fluid (IF) tubes. The lid contains 30 dehydrated substrates on tips of plastic prongs. The base has 30 reaction wells. Test inoculum is prepared with the inoculum fluid and is used to fill all 30 wells in the base. When the lid is aligned with the base and snapped in place, the test inoculum rehydrates the dried substrates and initiates test reactions.

Following an incubation period, the wells are examined for color changes. Color changes result from metabolic activities of the microorganisms. The resulting pattern of the 30 reactions is converted into a ten digit profile number that is used as the basis for identification.6 Biochemical and enzymatic reaction patterns for the 30 BBL Crystal E/NF ID substrates with a wide variety of microorganisms are stored in the BBL Crystal E/NF ID database. Identification is derived from a comparative analysis of the reaction pattern of the test isolate to those held in the database. A complete list of taxa that comprises the current E/NF database is provided in Table 1 (page 36).

User Quality Control: « User Quality Control:

Quality control testing is recommended for each lot of panels as follows –

1. Set up a BBL Crystal E/NF panel with Klebsiella pneumoniae ATCC™ 33495 per recommended procedure (refer to “Test Procedure”).

2. Incubate panel for 18 – 20 h at 35 – 37ºC.

3. Read panel with BBL Crystal Light Box or Panel Viewer and BBL Crystal E/NF Color Reaction Chart; record reactions using the BBL Crystal E/NF Report Pad. Alternatively, read the panel on the BBL Crystal AutoReader.

4. Compare recorded reactions with those listed in Table 2 (page 37). If discrepant results are obtained, confirm purity of quality control strain before contacting BD Technical Services.

Expected test results for additional quality control test strains are also listed in Table 2 (page 37).


Reagents: « Reagents:

The BBLCrystal E/NF ID panel contains 30 enzymatic and biochemical substrates as described below. Panel location indicates the row and column where the well is located (example: 1J refers to Row 1 in Column J).

Reagents and Principles of Tests Employed in the BBLCrystal E/NF ID System
Panel
Location		 Active
Ingredient		 Code Approx.
Amt.
(g/10 mL)		 Pos. Neg. Principle
(Reference)
4A Arabinose ARA 3.5 Gold/Yellow Orange/Red Utilization of carbohydrate results in lower pH and change in indicator (Phenol red).7-10
4B Mannose MNS 3.0 Gold/Yellow Orange/Red
4C Sucrose SUC 2.8 Gold/Yellow Orange/Red
4D Melibiose MEL 1.0 Gold/Yellow Orange/Red
4E Rhamnose RHA 3.0 Gold/Yellow Orange/Red
4F Sorbitol SOR 3.5 Gold/Yellow Orange/Red
4G Mannitol MNT 1.8 Gold/Yellow Orange/Red
4H Adonitol ADO 2.5 Gold/Yellow Orange/Red
4I Galactose GAL 1.5 Gold/Yellow Orange/Red
4J Inositol INO 1.3 Gold/Yellow Orange/Red
2A p-n-p-phosphate PHO 0.025 Yellow Colorless Enzymatic hydrolysis of the colorless aryl substituted glycoside or phospate ester releases yellow p-nitrophenol.1-5
2B p-n-p α-β-glucoside BGL 0.025 Yellow Colorless
2C p-n-p-β-galactoside NPG 0.06 Yellow Colorless
2D Proline nitroanilide PRO 0.07 Yellow Colorless Enzymatic hydrolysis of colorless amide substrate releases yellow p-nitroaniline.1-5
2E p-n-p bis-phosphate BPH 0.02 Yellow Colorless Enzymatic hydrolysis of the colorless aryl substituted glycoside or phospate ester releases yellow p-nitrophenol.1-5
2F p-n-p-xyloside BXY 0.03 Yellow Colorless
2G p-n-p-α-arabinoside AAR 0.03 Yellow Colorless
2H p-n-p-phosphorylcholine PHC 0.03 Yellow Colorless
2I p-n-p-β-glucuronide GLR 0.02 Yellow Colorless
2J p-n-p-N-acetyl glucosaminide NAG 0.04 Yellow Colorless
1A γ-L-glutamyl p-nitroanilide GGL 0.03 Yellow Colorless Enzymatic hydrolysis of the colorless amide substrate releases yellow p-nitroaniline.1-5
1B Esculin ESC 0.14 Brown/Maroon Clear/Straw Hydrolysis of esculin results in a black precipitate in the presence of ferric ion.11
1C p-nitro-DL-phenylalanine PHE 0.1 Gold/Dk. Orange Yellow Oxidative deamination of phenylalanine results in a brown color in the presence of ferric ion.7,11
1D Urea URE 0.2 Aqua/Blue Yellow/Green Hydrolysis of urea and the resulting ammonia change the pH indicator color (Bromthymol blue).7,11,12
1E Glycine GLY 0.7 Aqua/Blue Yellow/Green Degradation of glycine results in alkaline metabolites that change color of the pH indicator (Bromthymol blue).13
1F Citrate CIT 0.8 Aqua/Blue Yellow/Green Utilization of citrate results in alkaline metabolites that change color of the pH indicator (Bromthymol blue).7,14
1G Malonic acid MLO 1.5 Aqua/Blue Yellow/Green Utilization of malonate results in alkaline metabolites that change color of the pH indicator (Bromthymol blue).11
1H Triphenyl Tetrazolium chloride TTC 0.15 Pink/Red* Clear Reduction of the tetrazolium compound results in formation of a red formazan.13
1I Arginine ARG 1.5 Red/Purple Yellow/Brown Anaerobic catabolism results in pH rise and change in the color of the indicator (Bromcresol purple).7,15
1J Lysine LYS 0.5 Red/Purple Yellow/Brown
*Precipitate may or may not be visible.

After use, all infectious materials including plates, cotton swabs, inoculum tubes, filter papers used for oxidase or indole tests and BBL Crystal panels must be autoclaved prior to disposal or incinerated.


Warnings and Precautions: « Warnings and Precautions:

For in vitro Diagnostic Use



Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.