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BBL™ Crystal™ Anaerobe ID Kit

Cat. #	Description	Qty.	Unit
245010	BBL™ Crystal™ Anaerobe ID Kit Identifies clinically significant anaerobes in 4 hours. Separate databases for Schaedler Blood Agar, CDC Anaerobe Blood Agar and Alternate Blood Agars are included for improved specificity	20	SP

Intended Use:

The BBLCrystal™ Anaerobe (ANR) Identification (ID) System is a miniaturized identification method employing modified conventional, fluorogenic and chromogenic substrates. It is intended for the identification of frequently isolated anaerobic bacteria.^1-9

Catalog #	Description	Quantity	Unit
245010	BBL™ Crystal™ Anaerobe ID Kit Identifies clinically significant anaerobes in 4 hours. Separate databases for Schaedler Blood Agar, CDC Anaerobe Blood Agar and Alternate Blood Agars are included for improved specificity	20	SP

BBL Crystal™ Identification Systems
Anaerobe ID Kit
Revision: 2010/07 Revision Date: 07/01/2010

Intended Use:

Product Summary:

Micromethods for the biochemical identification of microorganisms were reported as early as 1918.¹⁰ Several publications reported on the use of the reagent-impregnated paper discs and micro-tube methods for differentiating enteric bacteria.^10-14 The interest in miniaturized identification systems led to the introduction of several commercial systems in the late 1960s, and they provided advantages in requiring little storage space, extended shelf life, standardized quality control and ease of use.

In general, many of the tests used in the BBLCrystal ID Systems are modifications of classical methods. These include tests for fermentation, oxidation, degradation and hydrolysis of various substrates. In addition, there are chromogen and fluorogen linked substrates, as in the BBLCrystal ANR ID panel, to detect enzymes that microbes use to metabolize various substrates.^12,15-22

The BBLCrystal ANR ID kit is comprised of (i) BBLCrystal ANR ID panel lids, (ii) BBLCrystal bases and (iii) BBLCrystal ANR, GP, RGP, N/H ID Inoculum Fluid (IF) tubes. The lid contains 29 dehydrated substrates and a fluorescence control on tips of plastic prongs. The base has 30 reaction wells. Test inoculum is prepared with the inoculum fluid and is used to fill all 30 wells in the base. When the lid is aligned with the base and snapped in place, the test inoculum rehydrates the dried substrates and initiates test reactions.

Following an incubation period, the wells are examined for color changes or presence of fluorescence that result from metabolic activities of the microorganisms. The resulting pattern of the 29 reactions is converted into a tendigit profile number that is used as the basis for identification.²³ Biochemical and enzymatic reaction patterns for the 29 BBLCrystal ANR ID substrates for a wide variety of microorganisms are stored in the BBLCrystal ANR ID data base. Identification is derived from a comparative analysis of the reaction pattern of the test isolate to those held in the database. A complete list of taxa that comprises the current database is provided in Table 1.

User Quality Control:

Quality control testing is recommended for each lot of panels as follows –

1. Inoculate a panel with Bacteroides fragilis ATCC™ 25285 per recommended procedure (refer to "Test Procedure").

2. Prior to incubation, let panel remain at room temperature for 1 min (not more than 2 min).

3. Read and record reactions with the aid of the panel viewer and color reaction chart.

4. If any of the wells, except 1F, are positive per color reaction chart (after 1 – 2 min), DO NOT USE PANELS from this lot. Contact BD Diagnostics Technical Services. (NOTE: Well 1F [Escosyl] should be positive upon rehydration.)

5. If all wells are negative, then incubate panel for 4 h at 35 – 37°C.

6. Read panel with the panel viewer and color reaction chart; record reactions using the Report Pad.

7. Compare recorded reactions with those listed in Table 4. If discrepant results are obtained, confirm purity of quality control strain before contacting BD Diagnostics Technical Services.

8. The incubator door should not be opened repeatedly during the incubation period (preferably less than 3 times).

Expected test results for additional quality control test strains are listed in Table 5.

Reagents:

The BBLCrystal ANR ID panel contains 29 enzymatic and biochemical substrates. Refer to table below for a list of active ingredients.

Table 3

Reagents used in the BBLCrystal ANR ID System


Panel Location	Substrate	Code	Pos.	Neg.	Active Ingredients	Approx. Amt. (g/L)

4A	Fluorescent negative control	FCT	n/a	n/a	Fluorescent coumarin derivative	≤1

2A	L-arginine-AMC	FAR	blue fluorescence > FCT well	blue fluorescence ≤ FCT well	L-arginine-AMC	≤1

1A	L-histidine-AMC	FHI	blue fluorescence > FCT well	blue fluorescence ≤ FCT well	L-histidine-AMC	≤1

4B	4MU-α-D-mannoside	FAM	blue fluorescence > FCT well	blue fluorescence ≤ FCT well	4MU-α-D-mannoside	≤1

2B	L-serine-AMC	FSE	blue fluorescence > FCT well	blue fluorescence ≤ FCT well	L-serine-AMC	≤1

1B	L-isoleucine-AMC	FIS	blue fluorescence > FCT well	blue fluorescence ≤ FCT well	L-isoleucine-AMC	≤1

4C	4MU-β-D-mannoside	FBM	blue fluorescence > FCT well	blue fluorescence ≤ FCT well	4MU-β-D-mannoside	≤1

2C	Glycine-AMC	FGL	blue fluorescence > FCT well	blue fluorescence ≤ FCT well	glycine-AMC	≤1

1C	L-alanine-AMC	FAL	blue fluorescence > FCT well	blue fluorescence ≤ FCT well	L-alanine-AMC	≤1

4D	4MU-N-acetyl-β-D-galactosaminide	FGA	blue fluorescence > FCT well	blue fluorescence ≤ FCT well	4MU-N-acetyl-β-D-galactosaminide	≤1

2D	L-pyroglutamic acid-AMC	FPY	blue fluorescence > FCT well	blue fluorescence ≤ FCT well	L-pyroglutamic acid-AMC	≤1

1D	L-lysine-AMC	FLY	blue fluorescence > FCT well	blue fluorescence ≤ FCT well	L-lysine-AMC	≤1

4E	L-methionine-AMC	FME	blue fluorescence > FCT well	blue fluorescence ≤ FCT well	L-methionine-AMC	≤1

2E	4MU-β-D-cellobiopyranoside	FCE	blue fluorescence > FCT well	blue fluorescence ≤ FCT well	4MU-β-D-cellobiopyranoside	≤1

1E	4MU-β-D-xyloside	FXY	blue fluorescence > FCT well	blue fluorescence ≤ FCT well	4MU-β-D-xyloside	≤1

4F	L-phenylalanine-AMC	FPH	blue fluorescence > FCT well	blue fluorescence ≤ FCT well	L-phenylalanine-AMC	≤1

2F	L-leucine-AMC	FLE	blue fluorescence > FCT well	blue fluorescence ≤ FCT well	L-leucine-AMC	≤1

1F	Escosyl*	FSC	blue/green fluorescence > FCT well	blue/green fluorescence ≤ FCT well	Escosyl	≤1

4G	Disaccharide	DIS	Gold/Yellow	Orange/Red	Disaccharide	≤300

2G	Furanose	FUR	Gold/Yellow	Orange/Red	Furanose	≤300

1G	Pyranose	PYO	Gold/Yellow	Orange/Red	Pyranose	≤300

4H	p-n-p-α-D-galactoside	AGA	Yellow	Colorless	p-n-p-α-D-galactoside	≤7

2H	p-n-p-β-D-galactoside	NPG	Yellow	Colorless	p-n-p-β-D-galactoside	≤7

1H	p-n-p-phosphate	PHO	Yellow	Colorless	p-n-p-phosphate	≤7

4I	p-n-p-α-D-glucoside	AGL	Yellow	Colorless	p-n-p-α-D-glucoside	≤7

2I	p-n-p-N-acetyl-glucosaminide	NAG	Yellow	Colorless	p-n-p-N-acetyl-glucosaminide	≤7

1I	L-proline-p-nitroanilide	PRO	Yellow	Colorless	L-proline-p-nitroanilide	≤7

4J	p-n-p-α-L-fucoside	AFU	Yellow	Colorless	p-n-p-α-L-fucoside	≤7

2J	p-n-p-β-D-glucoside	BGL	Yellow	Colorless	p-n-p-β-D-glucoside	≤7

1J	L-alanyl-L-alanine-p-nitroanilide	ALA	Yellow	Colorless	L-alanyl-L-alanine-p-nitroanilide	≤7

*The Escosyl substrate is fluorescent unhydrolyzed. Fluorescence will decrease when the enzyme is present.

Warnings and Precautions:

Precautions: in vitro Diagnostic

After review by the U.S. Centers for Disease Control and Prevention (CDC), and the Food and Drug Administration (FDA) under CLIA '88, this product has been identified as high complexity. The CDC Analyte Identifier Code is 0412; the CDC Test System Identifier Code is 07561.

After use, all infectious materials including plates, cotton swabs, inoculum tubes, filter papers used for indole tests and panels must be autoclaved prior to disposal or incinerated.

Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.

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