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BBL™ Crystal™ Anaerobe ID Kit
Cat. # Desc. Qty. Unit
245010 BBL™ Crystal™ Anaerobe ID Kit 20 SP
Intended Use:

The BBLCrystal™ Anaerobe (ANR) Identification (ID) System is a miniaturized identification method employing modified conventional, fluorogenic and chromogenic substrates. It is intended for the identification of frequently isolated anaerobic bacteria.1-9

Catalog # Description Quantity Unit
245010 BBL™ Crystal™ Anaerobe ID Kit
Identifies clinically significant anaerobes in 4 hours. Separate databases for Schaedler Blood Agar, CDC Anaerobe Blood Agar and Alternate Blood Agars are included for improved specificity
 20 SP

BBL Crystal™ Identification Systems
Anaerobe ID Kit
Revision: 0607    Revision Date: 06/01/2007   
Intended Use: «

The BBLCrystal™ Anaerobe (ANR) Identification (ID) System is a miniaturized identification method employing modified conventional, fluorogenic and chromogenic substrates. It is intended for the identification of frequently isolated anaerobic bacteria.1-9

Intended Use: » Intended Use:

The BBLCrystal™ Anaerobe (ANR) Identification (ID) System is a miniaturized identification method employing modified conventional, fluorogenic and chromogenic substrates. It is intended for the identification of frequently isolated anaerobic bacteria.1-9

Product Summary: «

Micromethods for the biochemical identification of microorganisms were reported as early as 1918.10 Several publications reported on the use of the reagent-impregnated paper discs and micro-tube methods for differentiating enteric bacteria.10-14 The interest in miniaturized identification systems led to the introduction of several commercial systems in the late 1960s, and they provided advantages in requiring little storage space, extended shelf life, standardized quality control and ease of use.

In general, many of the tests used in the BBLCrystal ID Systems are modifications of classical methods. These include tests for fermentation, oxidation, degradation and hydrolysis of various substrates. In addition, there are chromogen and fluorogen linked substrates, as in the BBLCrystal ANR ID panel, to detect enzymes that microbes use to metabolize various substrates.12,15-22

The BBLCrystal ANR ID kit is comprised of (i) BBLCrystal ANR ID panel lids, (ii) BBLCrystal bases and (iii) BBLCrystal ANR, GP, RGP, N/H ID Inoculum Fluid (IF) tubes. The lid contains 29 dehydrated substrates and a fluorescence control on tips of plastic prongs. The base has 30 reaction wells. Test inoculum is prepared with the inoculum fluid and is used to fill all 30 wells in the base. When the lid is aligned with the base and snapped in place, the test inoculum rehydrates the dried substrates and initiates test reactions.

Following an incubation period, the wells are examined for color changes or presence of fluorescence that result from metabolic activities of the microorganisms. The resulting pattern of the 29 reactions is converted into a tendigit profile number that is used as the basis for identification.23 Biochemical and enzymatic reaction patterns for the 29 BBLCrystal ANR ID substrates for a wide variety of microorganisms are stored in the BBLCrystal ANR ID data base. Identification is derived from a comparative analysis of the reaction pattern of the test isolate to those held in the database. A complete list of taxa that comprises the current database is provided in Table 1.

Product Summary: » Product Summary:

Micromethods for the biochemical identification of microorganisms were reported as early as 1918.10 Several publications reported on the use of the reagent-impregnated paper discs and micro-tube methods for differentiating enteric bacteria.10-14 The interest in miniaturized identification systems led to the introduction of several commercial systems in the late 1960s, and they provided advantages in requiring little storage space, extended shelf life, standardized quality control and ease of use.

In general, many of the tests used in the BBLCrystal ID Systems are modifications of classical methods. These include tests for fermentation, oxidation, degradation and hydrolysis of various substrates. In addition, there are chromogen and fluorogen linked substrates, as in the BBLCrystal ANR ID panel, to detect enzymes that microbes use to metabolize various substrates.12,15-22

The BBLCrystal ANR ID kit is comprised of (i) BBLCrystal ANR ID panel lids, (ii) BBLCrystal bases and (iii) BBLCrystal ANR, GP, RGP, N/H ID Inoculum Fluid (IF) tubes. The lid contains 29 dehydrated substrates and a fluorescence control on tips of plastic prongs. The base has 30 reaction wells. Test inoculum is prepared with the inoculum fluid and is used to fill all 30 wells in the base. When the lid is aligned with the base and snapped in place, the test inoculum rehydrates the dried substrates and initiates test reactions.

Following an incubation period, the wells are examined for color changes or presence of fluorescence that result from metabolic activities of the microorganisms. The resulting pattern of the 29 reactions is converted into a tendigit profile number that is used as the basis for identification.23 Biochemical and enzymatic reaction patterns for the 29 BBLCrystal ANR ID substrates for a wide variety of microorganisms are stored in the BBLCrystal ANR ID data base. Identification is derived from a comparative analysis of the reaction pattern of the test isolate to those held in the database. A complete list of taxa that comprises the current database is provided in Table 1.

User Quality Control: « User Quality Control:

Quality control testing is recommended for each lot of panels as follows –

1. Inoculate a panel with Bacteroides fragilis ATCC™ 25285 per recommended procedure (refer to “Test Procedure”).

2. Prior to incubation, let panel remain at room temperature for 1 min (not more than 2 min).

3. Read and record reactions with the aid of the panel viewer and color reaction chart.

4. If any of the wells, except 1F, are positive per color reaction chart (after 1 – 2 min), DO NOT USE PANELS from this lot. Contact BD Diagnostics Technical Services. (NOTE: Well 1F [Escosyl] should be positive upon rehydration.)

5. If all wells are negative, then incubate panel for 4 h at 35 – 37°C.

6. Read panel with the panel viewer and color reaction chart; record reactions using the Report Pad.

7. Compare recorded reactions with those listed in Table 4. If discrepant results are obtained, confirm purity of quality control strain before contacting BD Diagnostics Technical Services.

8. The incubator door should not be opened repeatedly during the incubation period (preferably less than 3 times).

Expected test results for additional quality control test strains are listed in Table 5.


Reagents: « Reagents:

The BBLCrystal ANR ID panel contains 29 enzymatic and biochemical substrates. Refer to table below for a list of active ingredients.

Table 3

Reagents used in the BBLCrystal ANR ID System

Panel Location Substrate Code Pos. Neg. Active Ingredients Approx. Amt. (g/L)
4A Fluorescent negative control FCT n/a n/a Fluorescent coumarin derivative ≤1
2A L-arginine-AMC FAR blue fluorescence
> FCT well		 blue fluorescence
≤ FCT well		 L-arginine-AMC ≤1
1A L-histidine-AMC FHI blue fluorescence
> FCT well		 blue fluorescence
≤ FCT well		 L-histidine-AMC ≤1
4B 4MU-α-D-mannoside FAM blue fluorescence
> FCT well		 blue fluorescence
≤ FCT well		 4MU-α-D-mannoside ≤1
2B L-serine-AMC FSE blue fluorescence
> FCT well		 blue fluorescence
≤ FCT well		 L-serine-AMC ≤1
1B L-isoleucine-AMC FIS blue fluorescence
> FCT well		 blue fluorescence
≤ FCT well		 L-isoleucine-AMC ≤1
4C 4MU-β-D-mannoside FBM blue fluorescence
> FCT well		 blue fluorescence
≤ FCT well		 4MU-β-D-mannoside ≤1
2C Glycine-AMC FGL blue fluorescence
> FCT well		 blue fluorescence
≤ FCT well		 glycine-AMC ≤1
1C L-alanine-AMC FAL blue fluorescence
> FCT well		 blue fluorescence
≤ FCT well		 L-alanine-AMC ≤1
4D 4MU-N-acetyl-β-D-galactosaminide FGA blue fluorescence
> FCT well		 blue fluorescence
≤ FCT well		 4MU-N-acetyl-β-D-galactosaminide ≤1
2D L-pyroglutamic acid-AMC FPY blue fluorescence
> FCT well		 blue fluorescence
≤ FCT well		 L-pyroglutamic acid-AMC ≤1
1D L-lysine-AMC FLY blue fluorescence
> FCT well		 blue fluorescence
≤ FCT well		 L-lysine-AMC ≤1
4E L-methionine-AMC FME blue fluorescence
> FCT well		 blue fluorescence
≤ FCT well		 L-methionine-AMC ≤1
2E 4MU-β-D-cellobiopyranoside FCE blue fluorescence
> FCT well		 blue fluorescence
≤ FCT well		 4MU-β-D-cellobiopyranoside ≤1
1E 4MU-β-D-xyloside FXY blue fluorescence
> FCT well		 blue fluorescence
≤ FCT well		 4MU-β-D-xyloside ≤1
4F L-phenylalanine-AMC FPH blue fluorescence
> FCT well		 blue fluorescence
≤ FCT well		 L-phenylalanine-AMC ≤1
2F L-leucine-AMC FLE blue fluorescence
> FCT well		 blue fluorescence
≤ FCT well		 L-leucine-AMC ≤1
1F Escosyl* FSC blue/green fluorescence
> FCT well		 blue/green fluorescence
≤ FCT well		 Escosyl ≤1
4G Disaccharide DIS Gold/Yellow Orange/Red Disaccharide ≤300
2G Furanose FUR Gold/Yellow Orange/Red Furanose ≤300
1G Pyranose PYO Gold/Yellow Orange/Red Pyranose ≤300
4H p-n-p-α-D-galactoside AGA Yellow Colorless p-n-p-α-D-galactoside ≤7
2H p-n-p-β-D-galactoside NPG Yellow Colorless p-n-p-β-D-galactoside ≤7
1H p-n-p-phosphate PHO Yellow Colorless p-n-p-phosphate ≤7
4I p-n-p-α-D-glucoside AGL Yellow Colorless p-n-p-α-D-glucoside ≤7
2I p-n-p-N-acetyl-glucosaminide NAG Yellow Colorless p-n-p-N-acetyl-glucosaminide ≤7
1I L-proline-p-nitroanilide PRO Yellow Colorless L-proline-p-nitroanilide ≤7
4J p-n-p-α-L-fucoside AFU Yellow Colorless p-n-p-α-L-fucoside ≤7
2J p-n-p-β-D-glucoside BGL Yellow Colorless p-n-p-β-D-glucoside ≤7
1J L-alanyl-L-alanine-p-nitroanilide ALA Yellow Colorless L-alanyl-L-alanine-p-nitroanilide ≤7

*The Escosyl substrate is fluorescent unhydrolyzed. Fluorescence will decrease when the enzyme is present.


Warnings and Precautions: « Warnings and Precautions:

Precautions: in vitro Diagnostic

After review by the U.S. Centers for Disease Control and Prevention (CDC), and the Food and Drug Administration (FDA) under CLIA ’88, this product has been identified as high complexity. The CDC Analyte Identifier Code is 0412; the CDC Test System Identifier Code is 07561.

After use, all infectious materials including plates, cotton swabs, inoculum tubes, filter papers used for indole tests and panels must be autoclaved prior to disposal or incinerated.



Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.