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BBL™ Crystal™ Neisseria/Haemophilus ID Kit
Cat. # Desc. Qty. Unit
245130 BBL™ Crystal™ Neisseria/Haemophilus ID Kit 20 SP
Intended Use:

The BBL Crystal™ Neisseria/Haemophilus (N/H) Identification (ID) system is a miniaturized identification method employing modified conventional, fluorogenic and chromogenic substrates. It is intended for the identification of frequently isolated Neisseria and Haemophilus as well as several other fastidious bacteria.1,2,6,15,18

Catalog # Description Quantity Unit
245130 BBL™ Crystal™ Neisseria/Haemophilus ID Kit
Identifies clinically significant Neisseria, Haemophilus and other fastidious organisms in 4 hours. The kit identifies 35 organisms from multiple media
 20 SP

BBL Crystal™ Identification Systems
Neisseria / Haemophilus ID Kit
Revision: 0607    Revision Date: 06/01/2007   
Intended Use: «

The BBL Crystal™ Neisseria/Haemophilus (N/H) Identification (ID) system is a miniaturized identification method employing modified conventional, fluorogenic and chromogenic substrates. It is intended for the identification of frequently isolated Neisseria and Haemophilus as well as several other fastidious bacteria.1,2,6,15,18

Intended Use: » Intended Use:

The BBL Crystal™ Neisseria/Haemophilus (N/H) Identification (ID) system is a miniaturized identification method employing modified conventional, fluorogenic and chromogenic substrates. It is intended for the identification of frequently isolated Neisseria and Haemophilus as well as several other fastidious bacteria.1,2,6,15,18

Product Summary: «

Micromethods for the biochemical identification of microorganisms were reported as early as 1918.3 Several publications reported on the use of the reagent-impregnated paper discs and micro-tube methods for differentiating enteric bacteria.3,4,8,19,21 The interest in miniaturized identification systems led to the introduction of several commercial systems in the late 1960s, and they provided advantages in requiring little storage space, extended shelf life, standardized quality control and ease of use.

In general, many of the tests used in the BBL Crystal ID Systems are modifications of classical methods. These include tests for fermentation, oxidation, degradation and hydrolysis of various substrates. In addition, there are chromogen and fluorogen linked substrates, as in the BBL Crystal N/H ID panel, to detect enzymes that microbes use to metabolize various substrates.5,6,8-10,13-17

The BBL Crystal N/H ID kit is comprised of (i) BBL Crystal N/H ID panel lids, (ii) BBL Crystal bases and (iii) BBL Crystal ANR, GP, RGP, N/H ID Inoculum Fluid (IF) tubes. The lid contains 29 dehydrated substrates and a fluorescence control on tips of plastic prongs. The base has 30 reaction wells. Test inoculum is prepared with the inoculum fluid and is used to fill all 30 wells in the base. When the lid is aligned with the base and snapped in place, the test inoculum rehydrates the dried substrates and initiates test reactions.

Following an incubation period, the wells are examined for color changes or presence of fluorescence that result from metabolic activities of the microorganisms. The resulting pattern of the 29 reactions is converted into a ten-digit profile number that is used as the basis for identification.20 Biochemical and enzymatic reaction patterns for the 29 BBL Crystal N/H ID substrates for a wide variety of microorganisms are stored in the BBL Crystal N/H ID data base. Identification is derived from a comparative analysis of the reaction pattern of the test isolate to those held in the database. A complete list of taxa that comprises the current database is provided in Table 1.

Product Summary: » Product Summary:

Micromethods for the biochemical identification of microorganisms were reported as early as 1918.3 Several publications reported on the use of the reagent-impregnated paper discs and micro-tube methods for differentiating enteric bacteria.3,4,8,19,21 The interest in miniaturized identification systems led to the introduction of several commercial systems in the late 1960s, and they provided advantages in requiring little storage space, extended shelf life, standardized quality control and ease of use.

In general, many of the tests used in the BBL Crystal ID Systems are modifications of classical methods. These include tests for fermentation, oxidation, degradation and hydrolysis of various substrates. In addition, there are chromogen and fluorogen linked substrates, as in the BBL Crystal N/H ID panel, to detect enzymes that microbes use to metabolize various substrates.5,6,8-10,13-17

The BBL Crystal N/H ID kit is comprised of (i) BBL Crystal N/H ID panel lids, (ii) BBL Crystal bases and (iii) BBL Crystal ANR, GP, RGP, N/H ID Inoculum Fluid (IF) tubes. The lid contains 29 dehydrated substrates and a fluorescence control on tips of plastic prongs. The base has 30 reaction wells. Test inoculum is prepared with the inoculum fluid and is used to fill all 30 wells in the base. When the lid is aligned with the base and snapped in place, the test inoculum rehydrates the dried substrates and initiates test reactions.

Following an incubation period, the wells are examined for color changes or presence of fluorescence that result from metabolic activities of the microorganisms. The resulting pattern of the 29 reactions is converted into a ten-digit profile number that is used as the basis for identification.20 Biochemical and enzymatic reaction patterns for the 29 BBL Crystal N/H ID substrates for a wide variety of microorganisms are stored in the BBL Crystal N/H ID data base. Identification is derived from a comparative analysis of the reaction pattern of the test isolate to those held in the database. A complete list of taxa that comprises the current database is provided in Table 1.

User Quality Control: « User Quality Control:

Quality control testing is recommended for each lot of panels as follows –

1. Inoculate a panel with Moraxella (Branhamella) catarrhalis ATCC™ 25240 per recommended procedure (refer to “Test Procedure”).

2. Prior to incubation, let panel remain at room temperature for 1 min (not more than 2 min).

3. Read and record reactions with the aid of the panel viewer and color reaction chart.

4. If any of the wells (except 1J) are positive per color reaction chart (after 1 – 2 min), DO NOT USE PANELS from this lot. Contact BD Technical Services.

5. If all wells are negative, then incubate panel for 4 h at 35 – 37°C.

6. Read panel with the panel viewer and color reaction chart; record reactions using the report pad.

7. Compare recorded reactions with those listed in Table 4. If discrepant results are obtained, confirm purity of quality control strain before contacting BD Technical Services.

8. The incubator door should not be opened repeatedly during the incubation period (preferably less than 3 times).

Expected test results for additional quality control test strains are listed in Table 5.


Reagents: « Reagents:

The BBLCrystal N/H ID panel contains 29 enzymatic and biochemical substrates. Refer to Table 3 for a list of active ingredients.

Table 3

Reagents used in the BBL Crystal N/H ID System

Panel
Location		 Substrate Code Pos. Neg. Active
Ingredients		 Approx.
Amt. (g/L)
4A Fluorescent negative control FCT n/a n/a Fluorescent coumarin
derivative		 ≤1
2A 4MU-phosphate FHO blue fluorescence
>FCT well		 blue fluorescence
≤FCT well		 4MU-phosphate ≤1
1A L-proline-AMC FPR blue fluorescence
>FCT well		 blue fluorescence
≤FCT well		 L-proline-AMC ≤1
4B L-serine-AMC FSE blue fluorescence
>FCT well		 blue fluorescence
≤FCT well		 L-serine-AMC ≤1
2B LYS-ALA-AMC FLA blue fluorescence
>FCT well		 blue fluorescence
≤FCT well		 LYS-ALA-AMC ≤1
1B L-tryptophan-AMC FTR blue fluorescence
>FCT well		 blue fluorescence
≤FCT well		 L-tryptophan-AMC ≤1
4C L-phenylalanine-AMC FPH blue fluorescence
>FCT well		 blue fluorescence
≤FCT well		 L-phenylalanine-AMC ≤1
2C N-succinyl-ALA-PRO-ALA-AMC FNS blue fluorescence
>FCT well		 blue fluorescence
≤FCT well		 N-succinyl-ALA-PRO-ALA-AMC ≤1
1C ALA-ALA-PHE-AMC FAA blue fluorescence
>FCT well		 blue fluorescence
≤FCT well		 ALA-ALA-PHE-AMC ≤1
4D L-glutamic acid-AMC FTA blue fluorescence
>FCT well		 blue fluorescence
≤FCT well		 L-glutamic acid-AMC ≤1
2D L-arginine-AMC FAR blue fluorescence
>FCT well		 blue fluorescence
≤FCT well		 L-arginine-AMC ≤1
1D Ornithine-AMC FOR blue fluorescence
>FCT well		 blue fluorescence
≤FCT well		 Ornithine-AMC ≤1
4E Glycine-AMC FGL blue fluorescence
>FCT well		 blue fluorescence
≤FCT well		 Glycine-AMC ≤1
2E GLY-PRO-AMC FGP blue fluorescence
>FCT well		 blue fluorescence
≤FCT well		 GLY-PRO-AMC ≤1
1E 4MU-β-D-galactoside FBG blue fluorescence
>FCT well		 blue fluorescence
≤FCT well		 4MU-β-D-galactoside ≤1
4F Saccharose SAC Gold/Yellow Orange/Red Saccharose ≤300
2F Maltotriose MTT Gold/Yellow Orange/Red Maltotriose ≤300
1F Carubinose CAR Gold/Yellow Orange/Red Carubinose ≤300
4G Pyranose PYO Gold/Yellow Orange/Red Pyranose ≤300
2G Maltobiose MTB Gold/Yellow Orange/Red Maltobiose ≤300
1G Disaccharide DIS Gold/Yellow Orange/Red Disaccharide ≤300
4H Riberol RBL Gold/Yellow Orange/Red Riberol ≤300
2H Levulose LEV Gold/Yellow Orange/Red Levulose ≤300
1H p-n-p-phosphorylcholine PHC Yellow Colorless p-n-p-phosphorylcholine ≤10
4I γ-L-glutamyl-p-nitroanilide GGL Yellow Colorless γ-L-glutamyl-p-nitroanilide ≤10
2I p-n-p-phosphate PHO Yellow Colorless p-n-p-phosphate ≤10
1I ONPG OPG Yellow Colorless ONPG ≤10
4J Urea URE Aqua/Blue Yellow/Green Urea ≤50
2J Resazurin REZ Pink Blue/Purple Resazurin ≤1
1J Ornithine ORN Purple Yellow/Gray Ornithine ≤200

Warnings and Precautions: « Warnings and Precautions:

Precautions: in vitro Diagnostic

After review by the Centers for Disease Control and Prevention (CDC), and the Food and Drug Administration (FDA) under CLIA ’88, this product has been identified as high complexity. The CDC Analyte Identifier Code is 0412; the CDC Test System Identifier Code is 07807.

After use, all infectious materials including plates, cotton swabs, inoculum fluid tubes, and panels must be autoclaved prior to disposal or incineration.



Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.