| Cat. # | Description | Qty. | Unit |
| 250160 | Directigen™ N. meningitidis Groups A, C, Y and W135 Kit
(90 determinations) containing: one 1 mL bottle N. meningitidis Groups C and W135 antibody, Reagent 1 , one 1 mL bottle N. meningitidis Groups A and Y antibody, Reagent 2 , one 3 mL bottle Positive Control , one 3 mL bottle Negative Control , one 0.5 mL bottle control latex Reagent A tab stirrers, plastic
|
1 | EA |
The Directigen™ N. meningitidis Groups A, C, Y and W135 Test is a presumptive latex slide agglutination test for the qualitative detection of antigens to Neisseria meningitidis Groups A, C, Y and W135 directly in cerebrospinal fluid (CSF), serum or urine. In addition, the test kit provides confirmation and serogrouping capabilities from suspected colonies of N. meningitidis Groups A/Y or C/W135. Visible agglutination occurs when a sample containing any of these bacterial antigens is reacted with its respective antibody-coated latex beads.
| Catalog # | Description | Quantity | Unit |
| 250160 |
Directigen™ N. meningitidis Groups A, C, Y and W135 Kit
(90 determinations) containing: one 1 mL bottle N. meningitidis Groups C and W135 antibody, Reagent 1 , one 1 mL bottle N. meningitidis Groups A and Y antibody, Reagent 2 , one 3 mL bottle Positive Control , one 3 mL bottle Negative Control , one 0.5 mL bottle control latex Reagent A tab stirrers, plastic
|
1 | EA |
The Directigen™ N. meningitidis Groups A, C, Y and W135 Test is a presumptive latex slide agglutination test for the qualitative detection of antigens to Neisseria meningitidis Groups A, C, Y and W135 directly in cerebrospinal fluid (CSF), serum or urine. In addition, the test kit provides confirmation and serogrouping capabilities from suspected colonies of N. meningitidis Groups A/Y or C/W135. Visible agglutination occurs when a sample containing any of these bacterial antigens is reacted with its respective antibody-coated latex beads.
The diagnosis of bacteremia and meningitis, especially in young children, can be difficult. As many as 55% of children are seen by a physician and started on antibiotics before meningitis is detected.1 Detection of microbial antigens in the CSF is a rapid and helpful method for diagnostic microbiology. It may be the single most important test in cases of partially treated meningitis since Gram stain and culture may be negative. Detection of a specific antigen is a clinically significant finding and a valuable aid when choosing antimicrobial therapy.2
Haemophilus influenzae type b, Neisseria meningitidis, and Streptococcus pneumoniae have been reported to be the three causative agents responsible for approximately 84% of cases of bacterial meningitis.3
Immunological methods for detecting characteristic exoantigens of pathogenic microorganisms in patient fluids (CSF, serum, urine) are typically faster than traditional methods such as culture. These techniques include counterimmunoelectrophoresis (CIE) and latex agglutination.4-7 The latex agglutination procedure has been found to be more rapid and sensitive than CIE in the detection of purified antigen.8,9
Include Control + and Control - testing with each batch of specimens tested as described in step 1, "Test Procedure."
Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.
| Reagent 1 | (1.0 mL), | Anti-N. meningitidis Groups C and W135, Rabbit Antibody-Coated Latex Suspension, with 0.2% sodium azide (preservative), |
| Reagent 2 | (1.0 mL), | Anti-N. meningitidis Groups A and Y, Rabbit Polyclonal Antibody-Coated Latex Suspension, with 0.2% sodium azide (preservative), |
| Reagent A | (0.5 mL), | Control Latex, Rabbit Immunoglobulin-Coated Latex Suspension, with 0.2% sodium azide (preservative), |
| Control + | (3.0 mL), | Polyvalent Positive Antigen Control, N. meningitidis Groups A/Y and C/W135, H. influenzae type b, S. pneumoniae, and Group B Streptococcus Antigens, with 0.2% sodium azide
(preservative), |
| Control – | (3.0 mL), | Negative Antigen Control, glycine buffered saline, with 0.2% sodium azide (preservative). |
For in vitro Diagnostic use.
Warning: Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"10-13 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. Prior to discarding, sterilize specimen containers and other contaminated materials by autoclaving.
Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.
