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For the detection of indole production as an aid in the identification of aerobic, anaerobic, or facultatively anaerobic organisms.¹

BBL™ DMACA Indole Reagent Droppers are used in a rapid spot test for determining the ability of bacteria to produce indole by the deamination of tryptophan. The production of indole has been widely used as an aid in differentiating certain genera and species within the same genus. p-Dimethylaminocinnamaldehyde (DMACA) has been shown to be sensitive to the production of indole.² In addition, the color change with DMACA is both rapid and long lasting, making results easier to interpret than with more traditional reagents.³

Each dropper is good for one day's use after the ampule has been broken.

Examine the reagent for signs of deterioration (See "Product Deterioration").

Positive (Escherichia coli ATCC™ 25922) and negative (Klebsiella pneumoniae ATCC 33495) controls should be performed along with the organisms to be tested.

Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent NCCLS guidance and CLIA regulations for appropriate Quality Control practices.

DMACA Indole Reagent Droppers contain 0.5 mL of aqueous 1% p-dimethylaminocinnamaldehyde in 10% hydrochloric acid.

For in vitro Diagnostic Use.

Follow proper laboratory procedures in handling and disposing of infectious materials. DMACA indole reagent is very acidic. Avoid contact with skin, eyes and mucous membranes. Rinse thoroughly with water if spilled.