United States
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 Nitrate A |
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| Cat. # | Desc. | Qty. | Unit |
| 261197 | Nitrate A | 50 | SP |
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BBL™ Nitrate Reagent A, BBL Nitrate Reagent B and BBL Nitrate Reagent C Droppers are for use in determining the ability of bacteria to reduce nitrate to nitrite or free nitrogen gas.
| Catalog # | Description | Quantity | Unit |
| 261197 | Nitrate A | 50 | SP |
BBL™ Nitrate Reagent A, BBL Nitrate Reagent B and BBL Nitrate Reagent C Droppers are for use in determining the ability of bacteria to reduce nitrate to nitrite or free nitrogen gas. BBL™ Nitrate Reagent A, BBL Nitrate Reagent B and BBL Nitrate Reagent C Droppers are for use in determining the ability of bacteria to reduce nitrate to nitrite or free nitrogen gas. The Enterobacteriaceae, many other gram-negative bacteria, mycobacteria and fungi reduce nitrate to nitrite. The nitrate-reducing characteristic of a species is constant for certain genera and species. The Nitrate A and B Reagents, when added in equal parts, indicate the presence of a catabolic end product or the absence of nitrate in the medium.1,2 In the event of a negative reaction, the addition of Nitrate C Reagent shows the presence of nitrate in the medium and confirms the negative reaction. The Enterobacteriaceae, many other gram-negative bacteria, mycobacteria and fungi reduce nitrate to nitrite. The nitrate-reducing characteristic of a species is constant for certain genera and species. The Nitrate A and B Reagents, when added in equal parts, indicate the presence of a catabolic end product or the absence of nitrate in the medium.1,2 In the event of a negative reaction, the addition of Nitrate C Reagent shows the presence of nitrate in the medium and confirms the negative reaction. Examine the reagents for signs of deterioration (see “Product Deterioration”). Escherichia coli ATCC™ 25922 will yield a positive result, while Acinetobacter baumannii ATCC 17961 will yield a negative result. Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices. Nitrate A Reagent Droppers contain 0.5 mL of 0.8% Sulfanilic Acid in 5N Acetic Acid. Nitrate B Reagent Droppers contain 0.5 mL of 0.6% N,N-Dimethyl-alpha-naphthylamine in 5N Acetic Acid. Nitrate C Reagent Droppers contain 1 g of zinc dust. For in vitro Diagnostic Use. Follow proper laboratory procedures in handling and disposing of infectious materials. Nitrate A Reagent causes burns. Keep locked up and out of reach of children. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing, gloves and eye/face protection. Nitrate B Reagent causes burns. When using, do not eat or drink. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing and eye/face protection. Nitrate B Reagent, although not classified as carcinogenic by the U.S. Occupational Safety and Health Administration (OSHA), is closely related to alpha-naphthylamine, which is considered a carcinogen.3 Nitrate C Reagent in contact with water liberates extremely flammable gases. Spontaneously flammable in air. Keep out of reach of children. Keep container tightly closed and dry. In case of fire, use sand, carbon dioxide or powdered extinguishing agent. Never use water. | |||||||||||
Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.
