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Methylene Blue Loeffler Reagent Dropper
Cat. # Desc. Qty. Unit
261204 Methylene Blue Loeffler Reagent Dropper 50 SP
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Intended Use: BBL™ Methylene Blue Loeffler Stain Droppers are used for presumptive identification of Corynebacterium diphtheriae and as an adjunct to the Gram stain.
Catalog # Description Quantity Unit
261204 Methylene Blue Loeffler Reagent Dropper 50 SP

Methylene Blue Loeffler Stain Droppers
Revision: 0703    Revision Date: 07/01/2003   

Intended Use: « BBL™ Methylene Blue Loeffler Stain Droppers are used for presumptive identification of Corynebacterium diphtheriae and as an adjunct to the Gram stain.
Intended Use: » Intended Use: BBL™ Methylene Blue Loeffler Stain Droppers are used for presumptive identification of Corynebacterium diphtheriae and as an adjunct to the Gram stain.

Product Summary: «

Methylene Blue is recognized as a simple stain that can be used for a variety of purposes, such as determining bacterial morphology and aiding in the interpretation of the Quellung test.1 It is the stain of choice for the presumptive identification of Corynebacterium diphtheriae. Upon staining, this species is characterized by a beaded or banded appearance of the cell, with deeply stained metachromatic granules contrasted with a lighter blue cytoplasm.2,3

Methylene Blue is used as an adjunct to the Gram stain, especially in the staining of gram-negative bacteria such as Haemophilus influenzae and Neisseria species.4,5 The stain can reveal the morphology of fusiform bacteria and spirochetes (from oral infections such as Vincent's angina) that may be difficult to see with the Gram stain.5 The stain is also used to detect the presence of fecal white blood cells that may show the presence of invasive bowel disease.5

BBL Methylene Blue Loeffler Stain Droppers can be used as a counterstain with BBL Flagella Stain Droppers, which are recommended for staining the flagella of bacteria.

BBL Methylene Blue Loeffler Stain Droppers are supplied in a hermetically sealed ampule for improved stability of the reagent prior to use.

Product Summary: » Product Summary:

Methylene Blue is recognized as a simple stain that can be used for a variety of purposes, such as determining bacterial morphology and aiding in the interpretation of the Quellung test.1 It is the stain of choice for the presumptive identification of Corynebacterium diphtheriae. Upon staining, this species is characterized by a beaded or banded appearance of the cell, with deeply stained metachromatic granules contrasted with a lighter blue cytoplasm.2,3

Methylene Blue is used as an adjunct to the Gram stain, especially in the staining of gram-negative bacteria such as Haemophilus influenzae and Neisseria species.4,5 The stain can reveal the morphology of fusiform bacteria and spirochetes (from oral infections such as Vincent's angina) that may be difficult to see with the Gram stain.5 The stain is also used to detect the presence of fecal white blood cells that may show the presence of invasive bowel disease.5

BBL Methylene Blue Loeffler Stain Droppers can be used as a counterstain with BBL Flagella Stain Droppers, which are recommended for staining the flagella of bacteria.

BBL Methylene Blue Loeffler Stain Droppers are supplied in a hermetically sealed ampule for improved stability of the reagent prior to use.


User Quality Control: « User Quality Control:

Identity Specifications – Blue, clear solution, inside a glass ampule contained in a plastic container.

Cultural Response – Test the performance of Methylene Blue Loeffler Stain Droppers. Observe C. diphtheriae for metachromatic granules.

Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent NCCLS guidance and CLIA regulations for appropriate Quality Control practices.

Organism ATCC™ Stain Reaction
Escherichia coli
Streptococcus pyogenes
Corynebacterium diphtheriae biotype gravis
25922
19615
8028
blue rods
blue cocci
deep blue granules

Reagents: « Reagents:

BBL Methylene Blue Loeffler Stain Droppers contain: 0.3 g Methylene Blue, 30 mL Alcohol, 95%, 0.1 mL Potassium Hydroxide, 10%, and 100 mL Distilled Water.


Warnings and Precautions: « Warnings and Precautions:

For in vitro Diagnostic Use.

Flammable. Harmful by inhalation and if swallowed. Keep container tightly closed. Avoid contact with skin and eyes. Wear suitable protective clothing.

Follow established laboratory procedures in handling and disposing of infectious materials.



Information shown on this page is a short summary extracted from the Package Insert, available as a PDF under the Related Documents section of this page.