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The Macro-Vue™ RPR (Rapid Plasma Reagin) 18 mm Circle Card Test is a nontreponemal testing procedure for the serologic detection of syphilis.^1,2

The Macro-Vue RPR Teardrop Card Test (using finger puncture blood) was the original Card Test and was developed for field use where testing could be performed without laboratory equipment.^3,4 By incorporating machine rotation, ringed test surfaces, and certain other technical changes, the RPR Circle Card Test was developed for use in large scale testing in public health and clinical laboratories.

The RPR 18 mm Circle Card Test is recommended when venous blood collection is employed and a large volume of serum is available, such as generally prevails in public health and clinical laboratories.^5-12 When a specimen contains antibody, flocculation occurs with a coagglutination of the carbon particles of the RPR Card antigen, which appear as black clumps against the white background of the plastic-coated card. By contrast, nonreactive specimens appear to have an even light-gray color.

In special situations when nontreponemal test results are needed rapidly and the specimen is collected as EDTA plasma, the RPR 18 mm Circle Card Test can be used if the test is performed within 24 h.^13,14

Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.

The ingredients* of the RPR Card antigen suspension are¹: 0.003% cardiolipin, 0.020 - 0.022% lecithin, 0.09% cholesterol, 0.0125 M EDTA, 0.01 M Na₂HPO₄, 0.01 M KH₂PO₄, 0.2% sodium azide (preservative), 0.02% charcoal (specially prepared, BD), 10% choline chloride, w/v, and deionized/distilled water.

*Adjusted and/or supplemented as required to meet performance criteria.

For in vitro Diagnostic Use.

Pathogenic microorganisms, including hepatitus viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"^15-18 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids.

Warning: RPR Card Antigen Suspension contains sodium azide, which is harmful if swallowed. Contact with acids liberates very toxic gas. After contact with skin, wash immediately with plenty of water. Sodium azide may react with lead and copper plumbing to form highly explosive metal azides. On disposal, flush with a large volume of water to prevent azide build-up.