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Trypticase™ Soy Agar (TSA) Isolator Pack plates are a general-purpose medium which supports the growth of a variety of bacteria and fungi. The Isolator Pack plates are particularly useful for microbial load testing when monitoring Isolator Systems, clean rooms, and other environmentally controlled areas when sterility of the medium is of importance.

The nutritional composition of the Trypticase Soy Agar base has made it a popular medium for many years. The medium is used for a multitude of purposes, including maintenance of stock cultures, aerobic microbial counts, isolation of microorganisms from a variety of specimen types and as a base for media containing blood.^1-3 It is included in the compendia of methods for the examination of water, waterwaste and foods.^4,5

The 100 x 15 mm and the 150 x 15 mm style plates can be used for active and passive air sampling.

Collection of samples from the same area before and after cleaning and treatment with a disinfectant permits the evaluation of the efficacy of sanitary procedures.

The multi-wrap design has been validated to protect the medium from vapor hydrogen peroxide when used in an Isolator System.

1. Examine plates for signs of deterioration as described under "Product Deterioration."

2. Check performance by inoculating a representative sample of plates with pure cultures of stable control organisms that give known, desired reactions. The following test strains are recommended:

For Laboratory Use.

Observe aseptic techniques and established precautions against microbiological hazards throughout all procedures. After use, prepared plates and other contaminated materials should be sterilized by autoclaving.