Product Center

Chopped Meat Carbohydrate Broth, PR II (pre-reduced medium in Hungate tubes) is a general-purpose medium for the enrichment and cultivation of anaerobic microorganisms, particularly obligate anaerobes.

Chopped Meat Carbohydrate Broth, PR II (pre-reduced) is a general enrichment broth medium that supports growth of most anaerobes, provides a backup source of culture material if an anaerobic jar or chamber fails and for enrichment of small numbers of organisms.¹ It may be used for subculture and enrichment of anaerobic isolates for chromatographic analysis and tests to determine proteolysis (meat digestion), spore formation, motility and toxin production, particularly by Clostridium species, and as a holding or stock culture maintenance medium.²

The use of Chopped Meat Carbohydrate Broth, PR II is based on Hungate's methods of culturing anaerobic microorganisms outside of an anaerobic chamber.³ The tubes provide a reduced medium in a self-contained, anaerobic culture tube sealed using a Hungate screw cap. The cap contains a butyl rubber septum stopper that permits inoculation and incubation without exposing the medium to air.

1. Examine tubes for signs of deterioration as described under "Product Deterioration".

2. Check performance by inoculating representative samples of tubes with pure cultures of stable control organisms that produce known, desired reactions. The following test strains are recommended:

Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent NCCLS guidance and CLIA regulations for appropriate Quality Control practices.

For in vitro Diagnostic Use

This product contains dry natural rubber.

Tubes with tight caps should be opened carefully to avoid injury due to breakage of glass.

Observe aseptic techniques and established precautions against microbiological hazards throughout all procedures. After use, prepared tubes, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.