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Egg Yolk Agar, Modified is a differential medium used in the isolation and presumptive differentiation of Clostridium species and other obligately anaerobic bacilli.

Egg Yolk Agar, Modified, is based on an egg yolk medium developed by McClung and Toabe for the isolation and presumptive differentiation of clostridia based on lecithinase and lipase production and proteolytic activity.¹

In this modification, CDC Anaerobe Agar (without vitamin K₁, which is subsequently added by egg yolks) is used as the basal medium instead of the McClung and Toabe formulation. CDC Anaerobe Agar is an enriched, nonselective medium that was developed at the Centers for Disease Control for use in the cultivation of obligately anaerobic microorganisms, particularly those found in clinical materials.²

CDC Anaerobe Agar is supplemented with egg yolk suspension for demonstration of lecithinase and lipase production and proteolytic activity.^1-7

See "Quality Control Procedures."

Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI guidance and CLIA regulations for appropriate Quality Control practices.

For in vitro Diagnostic Use.

If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.

Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"^8-11 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared plates, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding.