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Pseudosel™ Agar (Cetrimide Agar)

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Cat. # Description Qty. Unit
297882 Pseudosel™ Agar (Cetrimide Agar)
10 SP

Intended Use:

Pseudosel Agar (Cetrimide Agar) is used for the selective isolation and presumptive identification of Pseudomonas aeruginosa from clinical and nonclinical specimens.

Meets USP/EP/JP performance specifications, where applicable.1-3
Catalog # Description Quantity Unit
297882 Pseudosel™ Agar (Cetrimide Agar) 10 SP

BBL™ Pseudosel™ Agar (Cetrimide Agar)
Revision: 02     Revision Date: 08/01/2011    
Intended Use:

Pseudosel Agar (Cetrimide Agar) is used for the selective isolation and presumptive identification of Pseudomonas aeruginosa from clinical and nonclinical specimens.

Meets USP/EP/JP performance specifications, where applicable.1-3

Product Summary:

King et al. developed Medium A (Tech Agar) for the enhancement of pyocyanin production by Pseudomonas.4 Cetrimide (Pseudosel) Agar has the formula for Tech Agar but is modified by the addition of cetrimide (cetyl trimethyl ammonium bromide) for the selective inhibition of organisms other than P. aeruginosa.5

In 1951, Lowbury described the use of 0.1% cetrimide in a selective medium for P. aeruginosa.5 Because of the increased purity of the inhibitory agent, the concentration was later reduced, as reported by Lowbury and Collins in 1955.6 Brown and Lowbury employed incubation at 37°C with examination after 18 and 42 hours of incubation.7

Strains of P. aeruginosa are identified from specimens by their production of pyocyanin, a blue, water-soluble, nonfluorescent, phenazine pigment in addition to their colonial morphology8 and the characteristic grapelike odor of aminoacetophenone.9 P. aeruginosa is the only species of Pseudomonas or gram-negative rod known to excrete pyocyanin. Cetrimide (Pseudosel) Agar, therefore, is a valuable culture medium in the identification of this organism.

In addition to the promotion of pyocyanin production, Pseudosel Agar also enables the detection of fluorescent products produced by P. aeruginosa.

Cetrimide (Pseudosel) Agar is widely recommended for use in the examination of cosmetics,10 clinical specimens8,11 for the presence of P. aeruginosa, as well as for evaluating the efficacy of disinfectants against this organism.12 It is also used in the microbiological examination of nonsterile pharmaceutical products for Pseudomonas aeruginosa.1


User Quality Control:

See “Quality Control Procedures.”

Quality control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory’s standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI guidance and CLIA regulations for appropriate Quality Control practices.


Reagents:

Formula:
Pseudosel™ Agar
  Approximate Formula* Per Liter Purified Water
  Pancreatic Digest of Gelatin  20.0   g
  Potassium Sulfate  10.0   g
  Magnesium Chloride  1.4   g
  Agar  13.6   g
  Cetrimide  0.3   g
  Glycerol  10.0   mL
  *Adjusted and/or supplemented as required to meet performance criteria.

Warnings and Precautions:

For in vitro Diagnostic Use.

If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation.



Information shown on this page is a short summary extracted from the QC/PI Manual, available as a PDF under the Related Documents section of this page.