United States
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| Product Center | |
 HBT Bilayer Medium (for G. vaginalis) |
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| Cat. # | Desc. | Qty. | Unit |
| 297884 | HBT Bilayer Medium (for G. vaginalis) | 10 | SP |
HBT (Human Blood Tween) Bilayer Medium is a selective and differential medium used in the primary isolation and presumptive identification of Gardnerella vaginalis from clinical specimens.
| Catalog # | Description | Quantity | Unit |
| 297884 | HBT Bilayer Medium (for G. vaginalis) | 10 | SP |
HBT (Human Blood Tween) Bilayer Medium is a selective and differential medium used in the primary isolation and presumptive identification of Gardnerella vaginalis from clinical specimens. HBT (Human Blood Tween) Bilayer Medium is a selective and differential medium used in the primary isolation and presumptive identification of Gardnerella vaginalis from clinical specimens. HBT Bilayer Medium was described in 1982 by Totten et al. for the selective isolation and detection of G. vaginalis from clinical specimens.1 The basal medium consists of Columbia Agar supplemented with selective agents and Tween (polysorbate) 80. The top layer is made by adding human blood to the basal medium. Colonies of G. vaginalis may be differentiated by a diffuse, beta-hemolytic reaction they produce in the presence of human blood.2-5 Totten et al. reported that the rate of isolation of G. vaginalis was better on HBT Bilayer Medium than on a single layer human blood medium because the characteristic betahemolytic reaction used in differentiating the bacterium was more apparent.1 HBT Bilayer Medium was described in 1982 by Totten et al. for the selective isolation and detection of G. vaginalis from clinical specimens.1 The basal medium consists of Columbia Agar supplemented with selective agents and Tween (polysorbate) 80. The top layer is made by adding human blood to the basal medium. Colonies of G. vaginalis may be differentiated by a diffuse, beta-hemolytic reaction they produce in the presence of human blood.2-5 Totten et al. reported that the rate of isolation of G. vaginalis was better on HBT Bilayer Medium than on a single layer human blood medium because the characteristic betahemolytic reaction used in differentiating the bacterium was more apparent.1 See "Quality Control Procedures." Quality Control requirements must be performed in accordance with applicable local, state and/or federal regulations or accreditation requirements and your laboratory's standard Quality Control procedures. It is recommended that the user refer to pertinent CLSI (formerly NCCLS) guidance and CLIA regulations for appropriate Quality Control practices.
For in vitro Diagnostic Use. If excessive moisture is observed, invert the bottom over an off-set lid and allow to air dry in order to prevent formation of a seal between the top and bottom of the plate during incubation. Pathogenic microorganisms, including hepatitis viruses and Human Immunodeficiency Virus, may be present in clinical specimens. "Standard Precautions"6-9 and institutional guidelines should be followed in handling all items contaminated with blood and other body fluids. After use, prepared plates, specimen containers and other contaminated materials must be sterilized by autoclaving before discarding. Each donor unit of blood used in the preparation of this material was tested by an FDA approved method for the presence of the antibody to HIV (human immunodeficiency virus) and HBsAg (hepatitis B surface antigen) and found to be nonreactive. WARNING: POTENTIALLY BIOHAZARDOUS MATERIAL Because no test method can offer complete assurance that HIV, hepatitis B virus, or other infectious agents are absent, SPECIMENS AND THESE REAGENTS SHOULD BE HANDLED AS THOUGH CAPABLE OF TRANSMITTING AN INFECTIOUS DISEASE. The FDA recommends such material be handled at a Biosafety Level 2. Biosafety Level 2 is referenced in the Centers for Disease Control/National Institutes of Health manual, Biosafety in Microbiological and Biomedical Laboratories.8 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Information shown on this page is a short summary extracted from the QC/PI Manual, available as a PDF under the Related Documents section of this page.
