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Dehydrated Culture Media and Ingredients
Dehydrated Culture Media and Ingredients
BD supplies greater than 400 different Difco™ and BBL™ brand media formulations in dehydrated form for the convenience of the laboratorian and bioprocessing specialist.

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BD supplies greater than 400 different Difco™ and BBL™ brand media formulations in dehydrated form for the convenience of the laboratorian and bioprocessing specialist. Certain formulations are designated by a “II” in the medium name; these formulations are improved to provide more consistent, superior test results than earlier versions. The individual ingredients and the final dehydrated product are carefully quality-controlled to assure consistent, high quality performance and obviate the need for media to be prepared in the laboratory from raw materials. Dehydrated culture media may be offered as 100 g and 500 g bottles, 2 kg, 5 lb, 25 lb and 10 kg containers.

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Catalog # Description Brand Quantity Unit
244020 2xYT
Formulation designed for growth and propagation of E. coli infected with the single strand filamentous bacteriophage M13
 Difco 500 g EA
244010 2xYT
2xYT Formulation designed for growth and propagation of E. coli infected with the single strand filamentous bacteriophage M13
 Difco 2 kg EA
218231 A-1 Medium
Determination of fecal coliforms in water and foods
 Difco 500 g EA
231710 AC Broth
For cultivating a wide variety of microorganisms; for use in the sterility testing of turbid or viscous solutions and other materials not containing mercurial preservatives
 Difco 500 g EA
274210 Acetate Differential Agar
Differentiation of members of Shigella genus from Escherichia genus
 Difco 500 g EA
210920 Actinomyces Broth BBL 500 g EA
212168 Actinomycete Isolation Agar
Used with Glycerol. for isolation and cultivation actinomycetes from soil and water
 Difco 500 g EA
215710 Adonitol Difco 10 g EA
215810 Aesculin Difco 10 g EA
214050 Agar
A solidifying agent, is a purified agar in which extraneous matter, pigmented portions and salts are reduced to a minimum. It is available in the form of fine, light colored granules which are convenient for weighing and handling. It is usually used in concentrations of 1-2% in solid culture media or in concentrations of 0.05%-0.5% in media used for determination of motility and the growth of anaerobes and microaerophiles.
 Bacto 100 g EA
214040 Agar
A solidifying agent, is a purified agar in which extraneous matter, pigmented portions and salts are reduced to a minimum. It is available in the form of fine, light colored granules which are convenient for weighing and handling. It is usually used in concentrations of 1-2% in solid culture media or in concentrations of 0.05%-0.5% in media used for determination of motility and the growth of anaerobes and microaerophiles.
 Bacto 10 kg EA
214030 Agar
A solidifying agent, is a purified agar in which extraneous matter, pigmented portions and salts are reduced to a minimum. It is available in the form of fine, light colored granules which are convenient for weighing and handling. It is usually used in concentrations of 1-2% in solid culture media or in concentrations of 0.05%-0.5% in media used for determination of motility and the growth of anaerobes and microaerophiles.
 Bacto 2 kg EA
214010 Agar
A solidifying agent, is a purified agar in which extraneous matter, pigmented portions and salts are reduced to a minimum. It is available in the form of fine, light colored granules which are convenient for weighing and handling. It is usually used in concentrations of 1-2% in solid culture media or in concentrations of 0.05%-0.5% in media used for determination of motility and the growth of anaerobes and microaerophiles.
 Bacto 1 lb. EA
212304 Agar, Grade A BBL 454 g EA
214520 Agar, Granulated
Used as a solidifying agent for culture media. Carefully monitored for cultural response, solubility and gelation temperature
 Difco 10 kg EA
214530 Agar, Granulated
Used as a solidifying agent for culture media. Carefully monitored for cultural response, solubility and gelation temperature
 Difco 500 g EA
214510 Agar, Granulated
Used as a solidifying agent for culture media. Carefully monitored for cultural response, solubility and gelation temperature
 Difco 2 kg EA
214220 Agar, Noble
Solidifying agent essentially free of impurities used for electrophoretic and nutritional procedures wherever increased purity is required
 Difco 100 g EA
214230 Agar, Noble
Solidifying agent essentially free of impurities used for electrophoretic and nutritional procedures wherever increased purity is required
 Difco 500 g EA
299341 Agar, Select
Used as a solidifying agent for culture media. Carefully monitored for cultural response, solubility and gelation temperature
 BBL 5 lb. EA
299340 Agar, Select
Used as a solidifying agent for culture media. Carefully monitored for cultural response, solubility and gelation temperature
 BBL 500 g EA
281230 Agar, Technical
Solidifying agent for bacteriological culture media that do not have the exacting requirements as those for Bacto™ Agar. Carefully monitored for solubility, gelation temperature and other characteristics
 Difco 500 g EA
281210 Agar, Technical
Solidifying agent for bacteriological culture media that do not have the exacting requirements as those for Bacto™ Agar. Carefully monitored for solubility, gelation temperature and other characteristics
 Difco 2 kg EA
212272 Agarose
Used as a gelling agent for immunological applications
 BBL 500 g EA
210912 AK Agar #2 (Sporulating Agar) BBL 500 g EA
218151 Anaerobe Broth
MIC Susceptibility testing by the broth dilution technique
 Difco 500 g EA
253610 Anaerobic Agar
General purpose medium for anaerobic bacteria
 Difco 500 g EA
226340 Antibiotic Medium 1
Penassay Seed Agar Microbiological assay of antibiotics
 Difco 500 g EA
246310 Antibiotic Medium 10
Antibiotic Medium 10 Polymyxin Base Agar Microbiological assay of antibiotics
 Difco 500g EA
259310 Antibiotic Medium 11
Erythromycin/Neomycin Agar Microbiological assay of antibiotics
 Difco 500 g EA
266910 Antibiotic Medium 12
Microbiological assay of antibiotics
 Difco 500 g EA
210986 Antibiotic Medium 13
Sabouraud Liquid Broth Modified
 BBL 500 g EA
243100 Antibiotic Medium 19
Microbiological assay of antibiotics
 Difco 500 g EA
227020 Antibiotic Medium 2
Penassay Base Agar Microbiological assay of antibiotics
 Difco 500 g EA
224310 Antibiotic Medium 3
Penassay Broth
 Difco 2 kg EA
224320 Antibiotic Medium 3
Penassay Broth
 Difco 500 g EA
224410 Antibiotic Medium 4
Yeast Beef Agar Microbiological assay of antibiotics
 Difco 500 g EA
227710 Antibiotic Medium 5
Streptomycin Assay Agar Microbiological assay of antibiotics
 Difco 500 g EA
266710 Antibiotic Medium 8
Microbiological assay of antibiotics
 Difco 500 g EA
246210 Antibiotic Medium 9
Polymyxin Base Agar Microbiological assay of antibiotics
 Difco 500 g EA
233331 Antimicrobic Vial A Chlortetracycline, 10 mL
Used in the preparation of Bacto Cooke Rose Bengal Agar
 Difco 6 SP
233391 Antimicrobic Vial K Kanamycin, 10 mL
Used in the preparation of SFP Agar Base
 Difco 6 SP
232681 Antimicrobic Vial P Polymyxin B, 10 mL
Used in the preparation of MYP Agar and SFP Agar Base
 Difco 6 SP
265430 APT Agar
For cultivating heterofermentative lactobacilli and other organisms requiring high thiamine content
 Difco 500 g EA
265510 APT Broth
For cultivating heterofermentative lactobacilli and other organisms requiring high thiamine content
 Difco 500 g EA
214410 Asparagine Difco 500 g EA
240920 Azide Blood Agar Base
Isolation of streptococci and staphylococci; for use with blood in determining hemolytic reactions
 Difco 500 g EA
238710 Azide Dextrose Broth
Selective isolation of streptococci from water and sewage
 Difco 500 g EA
245710 B12 Assay Medium
For use in USP and AOAC methods for microbiological assay of Vitamin B12
 Difco 100 g EA
254110 B12 Culture Agar
To maintain cultures of Lactobacillus delbrueckii ATCC™ 7830 for use in USP and AOAC methods for microbiological assay of Vitamin B12
 Difco 100 g EA
254210 B12 Inoculum Broth
To prepare the inoculum of Lactobacillus delbrueckii ATCC™ 7830 for use in USP and AOAC methods for microbiological assay of Vitamin B12
 Difco 100 g EA
276810 Baird-Parker Agar Base
Use with EY Tellurite Enrichment for the detection and enumeration of coagulase positive staphylococci in foods and other materials
 Difco 2 kg EA
276840 Baird-Parker Agar Base
Use with EY Tellurite Enrichment for the detection and enumeration of coagulase positive staphylococci in foods and other materials
 Difco 500 g EA
214925 BBL™ CHROMagar™ Salmonella Difco 374 g EA
224710 BCG Dextrose Agar
Colorimetric estimation of lactobacilli in saliva
 Difco 500 g EA
212327 BCYE Agar Base BBL 500 g EA
211520 Beef Extract, Bacto™, Desiccated Dried form
Dried form Used to replace infusions of meat
 Difco 500 g EA
212303 Beef Extract, Powder BBL 500 g EA
271710 BG Sulfa Agar Brilliant Green
Selective isolation of Salmonella
 Difco 500 g EA
211027 BiGGY Agar
Bismuth Sulfite Glucose Glycine Yeast Agar is a selective and differential medium used in the detection, isolation and presumptive identification of Candida species
 BBL 500 g EA
299068 Bile Esculin Agar Base
Differential isolation and presumptive identification of group D streptococci
 BBL 500 g EA
213020 Bile Salts No. 3
Selective agent, inhibits gram-positive organisms
 Difco 500 g EA
213010 Bile Salts No. 3
Selective agent, inhibits gram-positive organisms
 Difco 100 g EA
241910 Biotin Assay Medium
For microbiological assay of biotin
 Difco 100 g EA
273300 Bismuth Sulfite Agar
For selective isolation of Salmonella, particularly Salmonella typhi, from food and clinical specimens
 Difco 500 g EA
248200 Bordet Gengou Agar Base
Enriched with defibrinated blood for the isolation of B. pertussis and other Bordetella species
 Difco 500 g EA
211968 Bovine Albumin
(Fraction V) 0.2% in 0.85% saline, 10 mL
 BBL 10 SP
266810 Bovine Albumin 5%, 20 mL Difco 12 SP
211057 Brain Heart CC Agar
For the isolation of pathogenic fungi from specimens heavily contaminated with bacteria and saprophytic fungi
 BBL 500 g EA
237200 Brain Heart Infusion
Cultivation of fastidious microorganisms, including streptococci, pneumococci and meningococci
 Bacto 2 kg EA
211060 Brain Heart Infusion
Cultivation of fastidious microorganisms, including streptococci, pneumococci and meningococci
 BBL 5 lb. EA
211059 Brain Heart Infusion
Cultivation of fastidious microorganisms, including streptococci, pneumococci and meningococci
 BBL 500 g EA
211061 Brain Heart Infusion
Cultivation of fastidious microorganisms, including streptococci, pneumococci and meningococci
 BBL 25 lb. EA
237500 Brain Heart Infusion
Cultivation of fastidious microorganisms, including streptococci, pneumococci and meningococci
 Bacto 500 g EA
237400 Brain Heart Infusion
Cultivation of fastidious microorganisms, including streptococci, pneumococci and meningococci
 Bacto 100 g EA
211065 Brain Heart Infusion Agar
Cultivation of fastidious microorganisms, especially fungi and yeasts; for use with added antibiotics in isolating fungi
 BBL 500 g EA
241820 Brain Heart Infusion Agar
Cultivation of fastidious microorganisms especially fungi and yeasts; for use with added antibiotics in isolating fungi
 Difco 100 g EA
241830 Brain Heart Infusion Agar
Cultivation of fastidious microorganisms especially fungi and yeasts; for use with added antibiotics in isolating fungi
 Difco 500 g EA
299069 Brain Heart Infusion Agar, Modified BBL 500 g EA
299070 Brain Heart Infusion Broth, Modified BBL 500 g EA
249910 Brain Heart Infusion with PAB and Agar
Modification of Brain Heart Infusion used for blood cultures, particularly with blood containing sulfonamides
 Difco 500 g EA
211069 Brain Heart Infusion with PABA BBL 500 g EA
256110 Brain Heart Infusion, Porcine
Cultivation of fastidious microorganisms using porcine as an alternate peptone source
 Bacto 10 kg EA
256120 Brain Heart Infusion, Porcine
Cultivation of fastidious microorganisms using porcine as an alternate peptone source
 Bacto 500 g EA
227920 Brewer Anaerobic Agar
Cultivation of anaerobic and microaerophilic organisms
 Difco 500 g EA
228530 Brilliant Green Agar Difco 500 g EA
218801 Brilliant Green Agar Modified (Edel-Kampelmacher)
Selective medium for the isolation of Salmonella from water, sewage and foodstuffs
 Difco 500 g EA
273000 Brilliant Green Bile 2%
Standard Methods medium for confirmed and completed tests for coliform bacteria in water and foods
 Difco 100 g EA
271000 Brilliant Green Bile 2%
Standard Methods medium for confirmed and completed tests for coliform bacteria in water and foods
 Difco 2 kg EA
274000 Brilliant Green Bile 2%
Standard Methods medium for confirmed and completed tests for coliform bacteria in water and foods
 Difco 500 g EA
214100 Brilliant Green Bile Agar
Selective differentiation of coliform bacteria
 Difco 500 g EA
211086 Brucella Agar BBL 500 g EA
211088 Brucella Broth BBL 500 g EA
216300 Buffered Glucose Broth
Methyl Red-Voges Proskauer Buffered Glucose Broth. Use with Difco™/BBL™ Voges-Proskauer Reagents A and B
 Difco 500 g EA
290720 Buffered Listeria Enrichment Broth Base
Used for the isolation of Listeria
 Difco 500 g EA
212367 Buffered Peptone Water BBL 500 g EA
212345 Buffered Peptone Water BBL 5 lb. EA
218403 Buffered Peptone Water II Preenrichment (discontinued)
For injured Salmonella in food
 		Difco 500 g EA
218401 Buffered Peptone Water II Preenrichment
For injured Salmonella in food
 Difco 2 kg EA
218103 Buffered Peptone Water Preenrichment
For injured Salmonella in food
 Difco 2 kg EA
218104 Buffered Peptone Water Preenrichment
For injured Salmonella in food
 Difco 10 kg EA
218105 Buffered Peptone Water Preenrichment
For injured Salmonella in food
 Difco 500 g EA
257820 Bushnell-Haas Broth
Cultivation and detection of bacteria capable of utilizing hydrocarbons
 Difco 500 g EA
214892 Campylobacter Agar Base
For use with blood and antibiotics in isolating and cultivating Campylobacter
 Difco 500 g EA
218201 Campylobacter Agar Base
For use with blood and antibiotics in isolating and cultivating Campylobacter
 Difco 2 kg EA
214891 Campylobacter Antimicrobic Supplement
(Vancomycin, Polymyxin B, Trimethoprim) Each vial when reconstituted to prepare 500 mL of Campylobacter Agar, Blaser formulation.
 Difco 6 SP
214890 Campylobacter Antimicrobic Supplement, Blaser , 5 mL
(Vancomycin, Polymyxin B, Trimethoprim, Cephalothin, Amphotericin B) Each vial when reconstituted to prepare 500 mL of Campylobacter Agar, Blaser formulation.
 Difco 6 SP
283510 Candida BCG Agar Base
For use with added neomycin for the primary isolation and identification of Candida sp.
 Difco 500 g EA
211102 Cary-Blair Transport Medium
For collecting, transporting and preserving microbiological specimens, particularly Vibrio cholerae
 BBL 500 g EA
211106 Casman Agar Base BBL 500 g EA
216010 Cellobiose (+), anhydrous, neither D nor L Difco 25 g EA
285420 Cetrimide Agar Base
Selective isolation and identification of P. aeruginosa
 Difco 500 g EA
211805 Chapman Stone Medium
Selective isolation of staphylococci based on mannitol fermentation and gelatinase activity
 Difco 500 g EA
289410 Charcoal Agar
Cultivation of fastidious organisms, particularly B. pertussis for vaccine production and stock culture maintenance
 Difco 500 g EA
245910 Chicken Pancreas
Desiccated, used in enzymatic liberation of folic acid from its conjugated state
 Difco 10 g EA
228950 Chocolate Agar Base (GC Medium)
Use with Hemoglobin or Hemoglobin 2%, Antimicrobic Vial CNV or CNVT, Supplement A, B, C or VX. Cultivation of N. gonorrhoeae and other fastidious organisms.
 Difco 500 g EA
228920 Chocolate Agar Base (GC Medium)
Use with Hemoglobin or Hemoglobin 2%, Antimicrobic Vial CNV or CNVT, Supplement A, B, C or VX. Cultivation of N. gonorrhoeae and other fastidious organisms.
 Difco 2 kg EA
228930 Chocolate Agar Base (GC Medium)
Use with Hemoglobin or Hemoglobin 2%, Antimicrobic Vial CNV or CNVT, Supplement A, B, C or VX. Cultivation of N. gonorrhoeae and other fastidious organisms.
 Difco 10 kg EA
246010 Choline Assay Medium
Microbiological assay of choline
 Difco 100 g EA
218172 CIN Agar Base, Modified
Use with Antimicrobic Supplement CN for selective isolation of Y. enterocolitica
 Difco 500 g EA
212218 CLED Agar (Cystine-Lactose-Electrolyte-Deficient Agar) BBL 500 g EA
211116 Coagulase Mannitol Agar BBL 500 g EA
211125 Columbia Agar
Base medium used with or without blood for cultivation of fastidious microorganisms
 BBL 5 lb. EA
211126 Columbia Agar
Base medium used with or without blood for cultivation of fastidious microorganisms
 BBL 25 lb. EA
211124 Columbia Agar
Base medium used with or without blood for cultivation of fastidious microorganisms
 BBL 500 g EA
279220 Columbia Blood Agar Base
Infusion free basal medium used with or without blood for cultivation of fastidious microorganisms
 Difco 2 kg EA
279240 Columbia Blood Agar Base
Infusion free basal medium used with or without blood for cultivation of fastidious microorganisms
 Difco 500 g EA
279230 Columbia Blood Agar Base
Infusion free basal medium used with or without blood for cultivation of fastidious microorganisms
 Difco 10 kg EA
279010 Columbia Blood Agar Base EH
Infusion-free basal medium used with blood for enhanced beta-hemolytic reactions after overnight incubation and for cultivation of fastidious microorganisms
 Difco 2 kg EA
279030 Columbia Blood Agar Base EH
Infusion-free basal medium used with blood for enhanced beta-hemolytic reactions after overnight incubation and for cultivation of fastidious microorganisms
 Difco 500 g EA
279020 Columbia Blood Agar Base EH
Infusion-free basal medium used with blood for enhanced beta-hemolytic reactions after overnight incubation and for cultivation of fastidious microorganisms
 Difco 10 kg EA
294420 Columbia Broth
Cultivation of fastidious microorganisms
 Difco 500 g EA
212249 Columbia CNA Agar
Use with blood for selective isolation of gram-positive cocci
 BBL 25 lb. EA
212104 Columbia CNA Agar
Use with blood for selective isolation of gram-positive cocci
 BBL 500 g EA
294221 Columbia CNA Agar
Use with blood for selective isolation of gram-positive cocci
 BBL 5 lb. EA
270310 Cooke Rose Bengal Agar
Use with or without Antimicrobic Vial A For the selective isolation of fungi from environmental and food specimens
 Difco 500 g EA
226730 Cooked Meat Medium
Cultivation of anaerobic bacteria and maintenance of stock cultures
 Difco 500 g EA
211132 Corn Meal Agar
General purpose medium for the cultivation of fungi. With the incorporation of polysorbate 80, utilized for the testing of Candida species for their ability to produce chlamydospores
 BBL 500 g EA
211094 CTA Agar BBL 500 g EA
211096 CTA Medium™ BBL 500 g EA
246710 Cystine Assay Medium
Microbiological assay of L-cystine
 Difco 100 g EA
252310 Cystine Tryptic Agar
Use with added carbohydrates in differentiating microorganisms based on fermentation reactions and motility
 Difco 500 g EA
233810 Czapek Dox Broth
Defined medium for cultivation of organisms capable of utilizing inorganic nitrogen
 Difco 500 g EA
233910 Czapek Solution Agar
Defined medium for cultivation of fungi and bacteria capable of utilizing inorganic nitrogen
 Difco 500 g EA
216310 D-Galactose (+), anhydrous Difco 500 g EA
217020 D-Mannitol Difco 500 g EA
217110 D-Mannose Difco 25 g EA
217910 D-Sorbitol Sorbite Difco 500 g EA
218110 D-Xylose Difco 25 g EA
268610 D/E (Dey/Engley) Neutralizing Agar
Testing of disinfectants and sanitation efficiency.
 Difco 10 kg EA
268620 D/E (Dey/Engley) Neutralizing Agar
Testing of disinfectants and sanitation efficiency
 Difco 500 g EA
281910 D/E (Dey/Engley) Neutralizing Broth
Testing of disinfectants and sanitation efficiency
 Difco 500 g EA
211144 DCLS Agar BBL 500 g EA
289020 Decarboxylase Base Moeller
Use to differentiate bacteria based on ability to decarboxylate amino acids
 Difco 500 g EA
287220 Decarboxylase Medium Base
Use with added amino acid in differentiating bacteria based on amino acid decarboxylation
 Difco 500 g EA
265320 Demi-Fraser Broth Base
For use with Fraser Broth Supplement in selectively and differentially enriching Listeria. Reduced Acriflavine and Nalidixic Acid levels
 Difco 500 g EA
265310 Demi-Fraser Broth Base
For use with Fraser Broth Supplement in selectively and differentially enriching Listeria. Reduced Acriflavine and Nalidixic Acid levels
 Difco 10 kg EA
212330 Dermatophyte Test Medium Base BBL 500 g EA
227310 Desoxycholate Agar
Differential selective medium for the isolation of gram-negative enteric bacilli
 Difco 500 g EA
227410 Desoxycholate Citrate Agar
Selective isolation of enteric pathogens, particularly Salmonella and Shigella
 Difco 500 g EA
242010 Desoxycholate Lactose Agar
For isolating and differentiating gram-negative enteric bacilli; for enumerating coliforms from water, wastewater, milk and dairy products
 Difco 500 g EA
267100 Dextrose Agar
Use with or without blood to cultivate a wide variety of microorganisms
 Difco 500 g EA
274720 Dextrose Agar Emmons
Modification of Sabouraud Dextrose Agar for the cultivation of pathogenic fungi
 Difco 500 g