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BD offers both traditional and cutting edge staining methodologies, as well as quality control slides for Gram stain and acid-fast staining procedures.

Within our new, state-of-the-art ISO 9000 manufacturing center is a dedicated production area for stain reagents. A unique BD manufacturing process reduces the presence of artifacts—a chemical precipitate sometimes mistaken for microorganisms in Gram stain preparations. The end result is easier to read and accurate staining. BD offers both traditional and cutting edge staining methodologies, as well as quality control slides for Gram stain and acid-fast staining procedures.
All Difco™/BBL™ stain kits are conveniently packaged in a compact tray and feature no-drip "keep clean" cap spouts, both on 250 mL bottles and economical gallon containers.

3-Step Gram Stain Kit
Combines decolorization and counterstaining into one step. This technology offers ease-of-use and reduces turn-around time by 25%.

4-Step Gram Stain Kit
Available with Stabilized/Unstabilized Iodine.

2-Step AFB Stain Kit
The TB Quick Stain Kit is a rapid 2-step modification of the Ziehl-Neelsen and Kinyoun techniques. No heating, no separate decolorization steps and no filtering required.

3-Step AFB Stain Kit
The AFB Staining product line includes the traditional Kinyoun or Ziehl-Neelsen Stains. Either stain enables the detection of acid-fast microorganisms without the use of a fluorescent microscope.

Fluorescent Stain Kits
BD advanced fluorescent stains are available in either the TB Fluorescent Kit M or our most popular TB Fluorescent Kit T, containing the Auramine-Rhodamine Stain. Rhodamine offers the benefit of reducing non-specific background fluorescence that may be present in either culture or clinical specimens.

Acridine Orange Stain
Acridine Orange Stain is used for detecting microorganisms in direct smears by fluorescence. It’s especially beneficial in screening normally sterile specimens where few organisms may be present. Bacteria stain bright orange; human cells, tissue debris and proteinaceous material stain pale green to yellow.

BBL™ QC Slides
Now there’s a safe, convenient and cost-effective method for performing quality control of Gram, acid-fast and fluorescent staining procedures. BBL™ Gram QC Slides and BBL™ AFB QC Slides eliminate the need for the laboratory staff to maintain stock QC cultures and prepare QC slides. In addition, these slides help standardize laboratory procedures.
BD BBL Gram QC Slides and BD BBL AFB QC Slides are just two more examples of new products created to meet the needs of the laboratory technician. BD continually adds to its continuum of products, with innovative product offerings in the areas of collection, rapid detection, isolation, identification and susceptibility testing.

BBL™ Gram QC Slides

• Conventional 1"x3" microscope slides imprinted with 10 squares.
• One square contains control organisms Staphylococcus aureus and Escherichia coli.
• Remaining nine squares are available for staining test isolates.
• Conventional 1"x3" microscope slides imprinted with two circles.

BBL™ AFB QC Slides

• The first circle is an acid-fast positive control with Staphylococcus aureus and Bacillus subtilis plus inactivated Mycobacterium tuberculosis H37Ra.
• The second circle is a negative control with an unstained mixture of non-acid-fast cocci (Staphylococcus aureus) and bacilli (Bacillus subtilis).