References

Journal Articles and Posters

• Comparative Human Plasma Peptidome Analysis: P100 vs EDTA
• Comparative Human Plasma Peptidome Analysis: P100 vs Serum
• AQUA Peptide Plasma and Serum Studies
• Mechanical Separator Studies
• Thrombin Studies: Nonspecific Proteolysis Induction in Serum Samples

• AQUA Peptide Plasma and Serum Studies

• Metabolic Peptide Biomarkers: From Sample to Spectrum

BD™ P100 Blood Collection System for Plasma Protein Preservation

Textbooks
Yi, J., Craft, D., Gelfand, CA., Methods Mol Biol. 2011; 728: 137-49. Minimizing preanalytical and analytical variability in plasma peptidomics using MALDI-TOF MS.

Comparative Human Plasma Peptidome Analysis: P100 vs EDTA

Journal Articles
J. Yi, et al.
"Inhibition of Intrinsic Proteolytic Activities Moderates Preanlaytical Variability and Instability of Human Plasma"
Journal of Proteome Research, 6:1768-1781 (2007). click here for a copy

Yi J, Liu Z, Craft D, O'Mullan P, Ju G, Gelfand CA.
"Intrinsic Peptidase Activity Causes a Sequential Multi-Step Reaction (SMSR) in Digestion of Human Plasma Peptides"
J Proteome Res., 2008 Dec;7(12):5112-8.

Craft D, Yi J, Gelfand CA.
"Time-Dependent and Sample-to-Sample Variations in Human Plasma Peptidome are Both Minimized Through Use of Protease Inhibitors"
Analytical Letters. 2009; 42(10): 1398-1406.

O'Mullan P, Craft D, Yi J, Gelfand CA.
"Thrombin induces broad spectrum proteolysis in human serum samples"
Clin Chem Lab Med. 2009;47(6):685-93.

• Preanalytical Variability of Plasma Samples and Mitigating Strategies
• Monitoring Peptidome Variance within Different Blood Collection to Further Understand Human Plasma Proteome Stability

• Stabilization of the Human Plasma Peptidome by Protease Inhibitors

• Using LC-MALDI-MS to Study the Variability and Stability of Plasma Proteins

• A Deeper Look Into Sample Collection and Handling-induced Variability of Plasma Proteins in Proteomics

• Characterization of Intrinsic Proteolytic Damage of Human Plasma Proteins During Sample Preparation  (print 11"x17" size)

• Preanalytical Variability and the Stability of Human Plasma Proteins: Mass Spectrometry Changes After Blood Collection (print 11"x17" size)

• Prevention of Proteolytic Digestion of Human Plasma Proteins by Protease Inhibitors

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Comparative Human Plasma Peptidome Analysis: P100 vs Serum

• Preanalytical Variability of Plasma Samples and Mitigating Strategies

• Inhibition of Intrinsic Proteolysis Stabilizes Plasma Samples

• Inhibition of Intrinsic Proteolytic Activity Moderates Preanalytical Variability and Stabilizes the Human Plasma Proteome

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AQUA Peptide Plasma and Serum Studies

• Demonstrating Instability of Peptide Biomarkers in Human Blood Plasma Using Time-Course Mass Spectrometry

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Mechanical Separator Studies

• Preanalytical Variability of Plasma Samples and Mitigating Strategies

• Effect of Plasma Separators on Peptide MALDI-TOF MS Spectra

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Thrombin Studies: Nonspecific Proteolysis Induction in Serum Samples

• Thrombin Exhibits Nonspecific Proteolysis in Serum; Observations with Fibrinopeptide A

• Intrinsic Preanalytical Variability of Serum Samples is Evidenced in Peptide MALDI MS Spectra

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• Demonstrating Instability of Peptide Biomarkers in Human Blood Plasma Using Time-Course Mass Spectrometry

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BD™ P800 Blood Collection System for Preservation of Plasma GLP-1, GIP, Glucagon and Ghrelin

Textbooks
Yi, J., Gelfand, CA., Craft, D., Methods Mol Biol. 2011; 728: 161-75. Investigation of peptide biomarker stability in plasma samples using time-course ms analysis.

• Metabolic Peptide Biomarkers: From Sample to Spectrum

• Ex vivo Stabilization of GLP-1 and GIP in Human Plasma
• Comparison of the BD P800 and BD P700 Blood Collection Systems and other Protease Inhibitors for the Preservation of Active GLP-1
• Compatibility of P800 with Insulin Testing for Research Purposes

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