BD ProEx™ C for ICC

BD ProEx™ C* is a highly sensitive and specific biomarker reagent used with standard immunocytochemistry (ICC) techniques to detect the presence of Aberrant S-Phase induction in routinely collected cytology specimens.

BD ProEx™ C is a protein-based biomarker reagent containing antibodies to MCM 2 protein and TOP2A. Increased expression of the MCM and TOP2A proteins are present in Aberrant S-Phase induction.

BD ProEx™ C slides are prepared using standard immunocytochemistry techniques performed manually or on an automated staining platform. The stained slides are evaluated qualitatively for the presence or absence of Aberrant S-phase induction.

Slide Preparation: A slide is prepared using a cytology specimen, and treated with a pretreatment buffer for target retrieval (e.g. BD SureDetect™ Slide Preparation Buffer).


Slide Staining: The slide is stained with BD ProEx™C and detection reagents and counterstains developed for use with mouse monoclonal antibodies in immunochemistry techniques. (e.g. BD SureDetect™ Detection Reagents and BD SureDetect™ Counterstains). The staining process can be performed either manually or with an automated staining platform (e.g. BD SMS 3600™ Molecular Stainer).


Slide Interpretation: Once stained and cover slipped, the slides are screened for the presence or absence of brown nuclear staining in cells, an indication of the presence or absence of Aberrant S-Phase induction.


Detecting Aberrant S-Phase Induction with the BD ProEx™ C ICC assay

In cells positive for aberrant S-phase induction, a number of proteins are over-expressed, including MCM2 and TOP2A. BD ProEx™ C reagent contains antibodies to MCM2 and TOP2A, which when added to the slide, forms antigen:antibody complexes. ICC based detection chemistry reagents are used to visualize these complexes. A DAB based chromogen imparts a brown nuclear stain to cells that are positive for aberrant S-phase induction.