BD ProEx™ C Reagent

BD ProEx™ C for ICC

BD ProEx™ C* is a highly sensitive and specific biomarker reagent used with standard immunocytochemistry (ICC) techniques to detect the presence of Aberrant S-Phase induction in routinely collected cytology specimens.

BD ProEx™ C is a protein-based biomarker reagent containing antibodies to MCM 2 protein and TOP2A. Increased expression of the MCM and TOP2A proteins are present in Aberrant S-Phase induction.

BD ProEx C slides are prepared using standard immunocytochemistry techniques performed manually or on an automated staining platform. The stained slides are evaluated qualitatively for the presence or absence of Aberrant S-phase induction.

Slide Preparation: A slide is prepared using a cytology specimen, and treated with a pretreatment buffer for target retrieval (e.g. BD SureDetect Slide Preparation Buffer).


Slide Staining: The slide is stained with BD ProExC and detection reagents and counterstains developed for use with mouse monoclonal antibodies in immunochemistry techniques. (e.g. BD SureDetect Detection Reagents and BD SureDetect Counterstains). The staining process can be performed either manually or with an automated staining platform (e.g. BD SMS 3600 Molecular Stainer).


Slide Interpretation: Once stained and cover slipped, the slides are screened for the presence or absence of brown nuclear staining in cells, an indication of the presence or absence of Aberrant S-Phase induction.


Detecting Aberrant S-Phase Induction with the BD ProEx™ C ICC assay

In cells positive for aberrant S-phase induction, a number of proteins are over-expressed, including MCM2 and TOP2A. BD ProEx C reagent contains antibodies to MCM2 and TOP2A, which when added to the slide, forms antigen:antibody complexes. ICC based detection chemistry reagents are used to visualize these complexes. A DAB based chromogen imparts a brown nuclear stain to cells that are positive for aberrant S-phase induction.