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Women's Health & Cancer

BD ProEx™ C Reagent

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BD ProEx™ C for IHC
BD ProEx™ C* is a highly sensitive and specific biomarker reagent used with standard immunohistochemistry (IHC) techniques to detect the presence of Aberrant S-Phase induction in routinely collected biopsy specimens.
BD ProEx™ C is a protein-based biomarker reagent containing antibodies to MCM 2 protein and TOP2A. Increased expression of the MCM and TOP2A proteins are present in Aberrant S-Phase induction.
ordering information
^{* Class 1 IHC (IVD)}

The BD ProEx™ C Process
BD ProEx™ C slides are prepared using standard immunohistochemistry techniques performed manually or on an automated staining platform. The stained slides are evaluated qualitatively for the presence or absence of Aberrant S-phase induction.

The BD ProEx™ C Process

Slide Preparation:	A paraffin-embedded formalin fixed tissue slide is prepared using a cytology specimen, and treated with a pretreatment buffer for target retrieval.
Slide Staining:	The slide is stained with BD ProEx™ C and detection reagents and counterstains developed for use with mouse monoclonal antibodies in immunochemistry techniques. The staining process can be performed either manually or with an automated staining platform.
Slide Interpretation:	Once stained and cover slipped, the slides are screened for the presence or absence of brown nuclear staining in cells, an indication of the presence or absence of Aberrant S-Phase induction.

Detecting Aberrant S-Phase Induction with the BD ProEx™ C IHC assay	In cells positive for Aberrant S-phase induction, a number of proteins are over-expressed, including MCM2 and TOP2A. BD ProEx™ C reagent contains antibodies to MCM2 and TOP2A, which when added to the slide, forms antigen:antibody complexes. IHC based detection chemistry reagents are used to visualize these complexes. A DAB based chromogen imparts a brown nuclear stain to cells that are positive for aberrant S-phase induction.

Detecting Aberrant S-Phase Induction
with Immunocytochemistry Assay

S-Phase Genes

Detecting Aberrant S-Phase Induction with Immunocytochemistry Assay

Detection

S-Phase Proteins

BD ProEx™ C antibodies

Reagents

DAB

Cell from patient with
Aberrant S-Phase Induction

¹	^{Kastan M. and Bartek J. Cell-Cycle Checkpoints and Cancer. Nature, 2004. Vol. 432: 316-323.}
²	^{Massague J. G1 Cell Cycle Control and Cancer. Nature. 2004. Vol. 432 : 298-306.}
³	^{Freeman A. et all. Minichromosome maintenance proteins as biological markers of dysplasia and malignancy. Clin Cancer Res. 1999; 5: 2121-2132.}