BD Vacutainer® CPT™ Cell Preparation Tube with Sodium Citrate

Test Principles

The BD Vacutainer® CPT™ Cell Preparation Tube with Sodium Citrate is an evacuated blood collection Tube system containing 0.1 M sodium citrate anticoagulant and blood separation media composed of a thixotropic polyester gel and a FICOLL™ HYPAQUE™ solution.

The bood separation media takes advantage of the relatively low density of mononuclear cells to isolate them from whole blood. The separation occurs during centrifugation when the gel portion of the medium moves to form a barrier separating the mononuclear cells and plasma from the denser blood components. The mononuclear cells can be collected by pipetting the cell layer, or the cells can be resuspended into the plasma by gentle inversion to improve cell viability if the sample is to be transported.

FICOLL is a trademark of Amersham Biosciences AB
HYPAQUE is a trademark of Amersham Health AS